Measurements should be repeatable, but since every laboratory has its own practices, instruments, and devices, the results of two repetitions of the same experiment are not always the same even though the same protocol is used. In this year’s InterLab study, iGEM teams transformed Escherichia coli DH5α with different plasmids expressing Green Fluorescent Protein (GFP) and a plate reader was used to measure absorbance and fluorescence values from the transformed strains. The results submitted by each team will be analyzed by the iGEM Measurement Committee, with the aim of evaluating the variability in measurements among different labs. The final goal would be to standardize the measuring procedures and increase their reproducibility.
The results obtained by our team are presented on this page. For all the steps, we used protocols provided by iGEM and did not encounter significant problems with them.