Team:BGU Israel/Attributions
Attributions
Overview
At the Ben-Gurion University, iGEM was recently established as a General University course under the auspices of the Faculty of Natural Sciences, Department of Life Sciences. The course is offered to students from all faculties in their last year of studies of their undergraduate degree. Only students that excel in their studies and have passed a personal interview with the course advisers are accepted and admitted to the course.
The course takes place over a full academic year, beginning in the academic year preceding the competition (Fall Semester) and ending after the iGEM Jamboree in the following Fall. The first assignment for the course was given during the summer vacation, when each student prepared an original idea for an iGEM project and presented it to the rest of the team. After all the ideas were presented, a team vote decided what will be the team’s choice project for that year. The next part of the course, which lasts approximately three months, involved intensive literature review and research to prepare a detailed research proposal. Each student thoroughly studied a specific element of the project and taught the information to the rest of the team. The last part of the course, involved lab work. The team established the lab themselves, ordered the necessary equipment and materials necessary for their project. From then on, the team has been working in the lab, applying their idea through experiments towards a proof of concept and demonstration of the viability of the proposed approach.
The goal of the iGEM course is to teach the students how to pursue independent scientific research. The students learn how to choose a research topic, whether it be a purely scientific question or one with practical applications. Then they learn how to choose experimental methods and plan experiments with the available equipment and knowledge, if necessary the team has consulted with experts in the field. Additionally, the students learn how to manage ongoing research through team meetings. The students experience real time problem solving, risk evaluation, writing research proposals, and experimental analysis.
In addition to the scientific aspect of the course, there is also the Human Practice aspect. Throughout the course the students work on presenting the project to the public through many different social and media platforms, as well as creating contacts with local and international researchers.
OriginALS Team
Mor Pasi
Ben Gurion University
Team leader, plasmid designer and synthesis, biobricks design and synthesis.
Mor Sela
Ben Gurion University
Astrocyte activation, promoter assays, cytokine validation via ELISA, collaboration, lab logistics.
Avital Bailen
Ben Gurion University
Human practices, plasmids preparation, qPCR experiments and Wiki content writer.
Sagi Angel
Ben Gurion University
Transfection experiments (calcium phosphate), plasmids mutagenesis, Interlab, social media and collaboration.
Einan Farhi
Ben Gurion University
Transfection experiments (electroporation), cell growth calibrations, cells selection, infection via lentivirus and genotyping.
Ori Tulchinsky
Ben Gurion University
Transfection experiments (reagents), calibrations and infection via lenti virus & AAV, packaging and patent writing and application.
Liat Tsoran
Ben Gurion University
Project idea, cells handling, infection via lenti virus and AAV, apoptosis assay, human practices, public media and IsrALS contact.
Nitzan Keidar
Ben Gurion University
Promoter assay experiments and promoters validation, plasmids preparation, human practices, Interlab and vlog content.
Daniel Deitch
Ben Gurion University
Plasmids preparation, patent, poster, presentation, animation, website design and content, team designer.
Supervisors and Instructors
Prof. Lital Alfonta
Ben Gurion University
Project management, scientific guidance and consulting, critical project evaluation, lab facility and equipment.
Dr. Ramon Birenbaum
Ben Gurion University
Project management, scientific guidance and consulting, critical project evaluation, lab facility and equipment.
Dror Aizik
Ben Gurion University
Scientific guidance, assistance in the design and implementation of experiments and protocols.
Dr. Orr Schlesinger
Ben Gurion University
Scientific guidance, assistance in the design and implementation of experiments and protocols.
Eden Ozer
Ben Gurion University
Scientific guidance, assistance in the design and implementation of experiments and protocols.
Naama Hirsch
Ben Gurion University
Scientific guidance, assistance in the design and implementation of experiments and protocols.
Project support and advice
Havi Zucker
Syntezza Bioscience Ltd.
Helped us design and order our synthetic plasmid
Dr. Emil Ruvinov
Ben Gurion University
As a former advisor of Boomerang iGEM team, helped with understanding iGEM and building the OriginALS project.
Vlad Sumeiko
Ben Gurion University
Helped with understanding iGEM, building the OriginALS project and provided us the platform of our electoronic notebook.
Shoham Rigbi
Ben Gurion University
Helped with understanding iGEM and building the OrigionALS project.
Dr. Dinorah
Friedmann-Morvinski
Tel Aviv University
Scientific advice, imaging and staining of reactive astrocytes, supplied plasmids for inhibition of Ikkb gene.
Dr. Adrian Israelson
Ben Gurion University
Scientific advice, help in project design, provide laboratory equipment, protocols, laboratory tools and cells for experiments.
Dr. Niva
Russek-Blum
Ben-Gurion University and DSASC
Information about the disease and consultation about the idea of the project.
Prof. Rachel Levy
Ben Gurion University
Information about the disease and consultation about the idea of the project.
Dr. Perry Nissen
Teva Pharmaceutical Industries Ltd.
Consulting and critical project evaluation.
Dr. Ayal Hendel
Bar Ilan University
Assisted us with choosing the correct Crispr system [MORE SPECIFIC].
Yuval Yugev
Ben-Gurion University of the Negev
Helped us to understand the benefits and limits of all the viral part in the project.
Research support
Dr. Kyoung Hwa Jung
Seoul National University
Answered technical questions about his experimental protocol and provided promoter sequences for IL1 and TNFa.
Dr. Young Gyu Chai
Hanyang University
Answered technical questions about his experimental protocol and provided promoter sequences for IL1 and TNFa.
Dr. Shane Liddelow
NYU Langone in New York City
Gave us advice on how to correctly activate astrocytes.
Research support
Dr. Michal
Shani-Sekler
Ben-Gurion University of The Negev
Laboratory manager, order equipment, conduct with university officials, organizing conference, organizing trip to US, fundraising
Dr. Uzi Hadad
Ben-Gurion University of The Negev
Helped us to use the FACS for the apoptosis assay. Instructed us in the use of an ImageStream device.
Dr. Dan Levi
Ben-Gurion University of The Negev
Allowed us the use of the illuminometer for promoter assay.
Dr. Ran Taube
Ben-Gurion University of The Negev
Let us use a fluorescent microscope for imaging.
Tatiana Rabinski
Ben-Gurion University of The Negev
Donated us supplies for mycoplasma testing when we were in need.
Difficult technique support
Yuval Yugev
Ben-Gurion University of The Negev
Tought us techniques for transfection, packaging , infection and virus concentration using Letivirus.
Dr. Joy Kahan
Ben-Gurion University of The Negev
Taught us techniques for transfection, package, infection and virus concentration using AAV.
Shirel Argueti
Ben-Gurion University of The Negev
Taught us to grow microglia BV2 cell line.
Reut Bar-Yaakov
Ben-Gurion University of The Negev
Taught us how to perform promoter assay.
Dr. Idit Dahan
Ben-Gurion University of The Negev
Helped with promoter assay, qPCR, and work with cells.
Dr. Dinorah
Friedman-Morvinski
Tel-Aviv University
Performed imaging by DAPI, Nestin and GFAP to C8D30 astrocyte cell line, and showed that our cell line is activated.
Inbar Angel
Tel-Aviv University
Answered technical questions about qPCR and virus infection.
Alina Abayev
Ben-Gurion University of The Negev
Instructed us how to do Electroporation to mammalian cells.
General support
Prof. Rivka Carmi
Ben-Gurion University of The Negev
President of Ben-Gurion University of the Negev.
Dr. Efrat Forti
Ben-Gurion University of The Negev
Gave us a full access to the Ilse Katz Institute Analytical facilities free of charge.
Provisional Patent Provisional Application
Dr. Assaf Geyra
Geyra Gassner Kesten, IP Law
Writing and submitting OriginALS provisional.
Revital Elihis
Geyra Gassner Kesten, IP Law
Writing and submitting OriginALS provisional.
Dr. Ora Horovitz
BG Negev commercialization entity
Commercialization and patent writing advice.
Website, Design and Animation
Ron Deitch
Israel Defensive Forces
Significant contribution to the programming and design of our WIKI.
Aviv Winter
Vikki Academy
Provided us a great service creating our project animation video.
Tomer Shary
Ben-Gurion University of the Negev
Introduced us to Vikki Academy animation company.
Tomer Doitshman
Cato Networks
Helped with the coding required to submit our BioBricks.
Human Practices support
Efrat Carmi
The Israel ALS Research Association
Took a significant part in organizing our scientific conference and in contacting researchers and students.
Manga Bar
Beer Sheva, Israel
Discounts for hosting the fundraising events at their pub.
Coca Bar
Beer Sheva, Israel
Discounts for hosting the “Happy-Hour” event after the ALS conference we organized.
Dr. Kim de Mora
Addgene
Facilitated podcast, advice about iGEM and provided discount for lab materials.
Dr. Joanne Kamens
Executive Director at Addgene
Facilitated podcast and provided discount for lab materials.
Dr. Tyler J. Ford
Addgene
Podcast editor.
Osnat Eitan
Ben Gurion University
Director, Department of Publications and Media Relations BGU
Dani Machlis
Ben Gurion University
BGU photographer.
Naama Mishaly
Galay PR
Public relations.
Amir Rozenblit
Ben Gurion University
Press Officer & Spokesperson
Andy Friedman
Ben-Gurion University of the Negev
US press.
Special thanks and donations
Kevin M. Leopold
American Associates
Ben-Gurion University of the Negev
Facilitated meetings with sponsors and press in the US.
Chani Pearlstein
American Associates
Ben-Gurion University of the Negev
Facilitated meetings with sponsors and press in the US.
Sponsors
Prof. Rivka Carmi
Ben-Gurion University of the Negev
President of Ben-Gurion University of the Negev.
Prof. Zvi Hacohen
Ben-Gurion University of the Negev
Rector of Ben-Gurion University of the Negev.
Prof. Michal Shapira
Ben-Gurion University of the Negev.
Dean of the Faculty of Natural Sciences
Prof. Amos Katz
Ben-Gurion University of the Negev
Dean of the Faculty of Health Sciences.
Prof. Avraham Levi
Ben-Gurion University of the Negev
Dean of the Faculty of Engineering Sciences.
IKI
Ilse Katz Institute
for Nanoscale Science & Technology
Donations.
Integrated
DNA Technologies
Donations.
IsrALS
The Israel ALS Research Association
Donations.
Lumitron
Donations.
Labotal
Donations.
Biological
Industries
Donations.
MERCK
Donations.
ZOTAL
Donations.
RHENIUM
Donations.
Kirur-Benny
Donations.
A2S
Donations.
Tivan Biotech
Donations.
Bio-Rad
Donations.
Danyel BIOTECH
Donations.
Promega
Biological Industries
Donations
BioAnalytics
Donations.