Team:BIT/Notebook

<!DOCTYPE html> Notebook

NOTEBOOK

Stage 1(3.26-6.10)

In Last selection of members
Out We have a meet-up with team ZJUT-CHINA in our school
Click here for more

In iGEM_BIT was established,then we started discussing about our directions of project for this year. Team members were divided into 3 groups. Then was a 3-week-long brainstorm.

In A simple presentation of Interim work from 3 groups.
Out We invited our Primary Instructor, Xuefei Lv, to participate in our discuss meeting.

In A final investigate and survey presentation of from 3 groups. Through Voting, team members selected the project that they think is the best.
Out This week,we invited Pro. Deng Yulin together with our PI Xuefei Lv ,with their review and our discussion , we determined the primary direction of our project.Interview of Pro.Deng about our project

In We moved on to the next step. And the hold team were focusing on the project and making deeper investigation and survey.
Out We want to know how others thought about our JACOB2.0, so we start an online survey trying to collect results. Results will be integrated and analysed in week 7

In Before going into the laboratory, All team members had several laboratory safety training lessons. And nobody have the right to go into the laboratory if he/she didn’t get permission from teacher Yaxi Zhang, who is responsible for the lab safety. In this week, we discussed about the method that might use in biological experiments. And completed reagent selection and purchase also.
Out survey on going still

In We studied Genetic engineering experiments like Plasmid extraction and transformation, and practice our experimental skills
Out survey on going still

In Kept studying and doing experiments
Microfluidic chip design started
Out We collected results of our survey and have conclusions about it. Click here for more information

In We did preliminary biology experiments.
We also studied PDMS microfluidic chip making
Out We went to the clinical laboratory department of aerospace general hospital in Beijing and learned about the related detection method of biomarkers. Click here for more

Stage 2(6.11-9.23)

In We did affinity validation between CKMB protein and aptamer We did experiments of gene circuit 2 connections (inverse ratio line) We studied PDMS microfluidic chip making
Out We have a meet-up with team SDU-CHINA in our school. Click here for more details.

In We did affinity validation between CKMB protein and aptamer We did experiments of gene circuit 2 connections (inverse ratio line) three times We started writing safety form carefully. And we participated in the Interlab study. We studied PDMS microfluidic chip making
Out We conducted interviews of local people in remote areas of Guizhou province. Click here for more

In We did affinity validation between CKMB protein and aptamer together experiments of gene circuit 2 connections (inverse ratio line) Kept studying PDMS microfluidic chip making
Out We have communication with team ZJUT-China about helping them with their biology work.Click here for more

In We did experiments of competitive binding verification of CKMB protein and aptamer, then did interlab experiments of requirement 1 and got some result. Finished making first edition of chip.And tested its’ function, unfortunately, this edition can not meet our need.
Out In order to make sure our design is we went to XXX company, Click here for more information.

In We did experiments of competitive binding verification of CKMB protein and aptamer We finished requirement 1 of Interlab. Converting between absorbance of cells to absorbance of a known concentration of beads. Microfluidic chip redesign started
Out Online communication with XJTLU team.Click here for more

In We did experiments of competitive binding verification of CKMB protein and aptamer We finished requirement 2 Counting colony-forming units (CFUs) from the sample. We made data processing also. Modeling survey started Chip making started
Out We have communicated with CCU_Taiwan through Skype and spent two meaningful hours together. Click here for more information.

In did experiments of competitive binding verification of CKMB protein and aptamer modeling survey started We submitted our data for Interlab study. Finished making second edition of chip.And test result is not very good also.So we start learning to make PMMA microfluidic chips in order to make our chip suitable for the hole project.
Out Interview of Pro.Deng about our project. Click here for more

In did experiments of competitive binding verification of CKMB protein and aptamer modeling survey keep going,and collect some results did experiments of gene circuit 1 connections Kept learning making PMMA microfluidic chips
Out We contact a professor in School of Mechanical Engineering in our university. He gives us really great advice about how to design our holding apparatus for optical instruments.

In did experiments of gene circuit 1 connections We tested response of different concentrations of SAM to the circuit Kept learning making PMMA microfluidic chips,finfished the third edition of chip
Out We communicated about our biology design with team XMU-China. Click here for more

In We tested response of different concentrations of SAM to the circuit We tested function of chip.It turned out good result. Device design started
Out we went to BOHUI-Tech to investigate about POCT device and share ideas with them. Click here for more information

In We tested response of different concentrations of SAM to the circuit, After 3 weeks test and result shown. We found that this method can not meet one of our ultimate goal, quantitative determination. So we are trying to optimize our current methods again. So we discussed and tried to find methods to solve this problem. After discussing, we gave a primary plan.

In Design experiments and purchase reagents. Complete and check our safety form, then submitted it.

In Preliminary verify the experimental design (aptamer competition and affinity experiment) Device simulation diagram making.
Out Middle school preaching and teaching. Click here for more

In Flow Cytometric Analysis of the connection between complementary chains and bacteria Component purchase of device
Out preaching for the freshmen in BIT.Click here for more

In did experiments: linking beads to aptamers, linking aptamers to complementary chains, competitive experiment of protein sample biomarker with complementary chain for aptamer. device assembly
Out High/middle school preaching and teaching. Click here for more

Stage 3(9.24-10.16)

Repeated last week's experiments
presentation practice
Building and filling our wiki page

Filling in our story on wiki page.
Completed of the most important deliverable carefully--judging form.
Presentation practice again and again
Poster design and making

Checking our wiki pages and correct small mistakes or fix the bug
Then wiki freeze!!!