till the known mineable gold ressources are depleted1
till the known mineable silver ressources are depleted2
We were in close contact and in a continuous dialogue with a lot of scientific experts and stakeholders associated with our project. This Integrated Human Practice shaped our project and helped us to gain a lot of expertise.
We improved the human ferritin, delivered by iGEM Calgary 2013, by mutating certain amino acid residues to change the affinity towards iron to an improved affinity for gold and silver.
We modeled to get an impression on the toxicity caused by metal ions to design our ROS reduction system based on this model. Furthermore, we modeled certain process parameters to improve our hardware.
During our project we were able to accumulate metal ions in Escherichia coli, while reducing the toxicity. We engineered ferritin to enable iron, silver and gold nanoparticle formation. Furthermore, we showed that nanoparticles could be used to print conductive circuits.
We submitted and characterized our copper importer CopD (BBa_K2638002) to work as expected.
We collaborated with great iGEM teams, learned a lot through our mentoring of two teams and got a lot of feedback on our Outreach.
1 Quote from Eugene King, analyst from Goldman Sachs2 Sverdrup, H., Koca, D., & Ragnarsdottir, K. V. (2014). Investigating the sustainability of the global silver supply, reserves, stocks in society and market price using different approaches. Resources, Conservation & Recycling, 83, 121-140. DOI: 10.1016/j.resconrec.2013.12.008
3 Andreas Manhart, Markus Blepp, Corinna Fischer, Kathrin Graulich, Siddharth Prakash, Rasmus Priess, Tobias Schleicher, Maria Tür (2016). Resource Efficiency in the ICT Sector Final Report.