Team:Bielefeld-CeBiTec/Experiments

Experiments

Protocol	Protocol
“Typical” Restriction Digest	50x TAE Stock Solution
Blunt end ligation	Cloning Strategy with Restriction Enzymes
Colony PCR GoTaq® G2 DNA Polymerase	Fast cell lysis for SDS-Page
Generating chemocompetent cells	Generating electrocompetent cells
Genome editing via the CRISPR-Cas9 system	Gibson Assembly
IMPACT-CN	innuPREP Gel Extraction Kit from Analytik Jena
Lenox Broth (LB)	Maldi
PCR with Phusion High-Fidelity PCR Master Mix with GC Buffer	Plasmid Assembly Protocol with Golden Gate Assembly
Produce gold in Ferritin	PureYieldTM Plasmid Miniprep System from Promega
ssDNA Annealing	Standard BioBrick Assembly
TAE buffer	ThermoFischer Scientific Gel Extraction GenJet
Transformation via electroporation	Transformation via heat shock
Western Blot	Wizard SV Gel and PCR Clean-Up Promega
ZymoPURETM Plasmid Miniprep Kit from Zymo Research	Silver nanoparticle synthesis
Phytochelatin assay	ONPG membrane permeability assay
Remove Iron of Ferritin	NPN assay
Measurement of the phytochelatin synthase assay	Cultivation for Expression of recombinant proteins
Roti-Nanoquant	Ferritin Purification
Roti-Nanoquant(for protein concentration estimation)	SDS-PAGE
SDS-PAGE running buffer

Enzyme