Team:Bio Without Borders/Safety

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• H. PRACTICES

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• JUDGING FORM

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Safe Project Design

Since our team had never before competed in iGEM, we decided to stick to very safe species: horseshoe crabs, E. coli K12 strains, and the industrial yeast Pichia pastoris for our project.

• For all cloning we chose non-pathogenic chassis organisms
• We chose to express single proteins rather than pathways and focus on diagnostics rather than therapeutics
• For our proof-of-concept experiment we used E.coli K12 rather than native E. coli as a source of endotoxins

Safe Lab Work

We wore personal protective gear appropriate to the organisms and chemicals we worked with, and were careful to clean up after experiments with 70% ethanol on the benchtops. when we had to transport our Pichia pastoris to Columbia University for electroporation, we carried it in capped tubes sealed in a styrofoam box.

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