BostonU 2018 is characterizing and optimizing a pair of light-inducible promoters, LOV2 and PhiReX, in S. cerevisiae for use in eukaryotic transcriptional control and in industrial fermentation. Light-inducible promoters lend synthetic biologists greater spatiotemporal control over transcription than small molecule-inducible promoters, and represent an expansion of tools for transcriptional control. Further, LOV2 is activated by blue light and PhiReX by red, allowing for multiple layers of control. To characterize these light-inducible promoter systems, BostonU is using the eVOLVER, a novel cell culturing platform developed by Brandon Wong at Boston University’s Khalil Lab. The eVOLVER allows us to fully characterize our systems across an expansive parameter space, as well as multiplex across strains while retaining control of individual cultures.
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