Dispersin B is a β-hexosaminidase that specifically hydrolyzes β-1,6-glycosidic linkages of acetylglucosamine polymers found in biofilm. This enzyme is derived from Aggregatibacter actinomycetemcomitans, which secretes adherent cells from mature biofilm colonies by secreting disperse B. The active site of Disperse B contains three highly conserved acidic residues: aspartic acid at residue 183 (D183), glutamic acid at residue 184 (E184), and glutamic acid at residue 332 (E332).
We hope that DspB, like siderophore, is regulated by AHL, and when AHL is present, the dspb gene will express. Given that the DspB enzyme activity is low, we planed to make it accumulate in cells first, and then be released by the subsequent lysis system.
In order to test the biofilm removal effect of DspB, we constructed the vector pET28a-dspB.
1. Nature Communications 11 April 2017 Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models in Young Hwang
2. Biotechnological Products and Process Engineering 23 July 2004 Inhibiting mild steel corrosion from sulfate-reducing and iron-oxidizing bacteria using gramicidin-S-producing biofilms