We constructed a quorum-sensing system and found that the promoter with afe-box can work when the AHL concentration is more than 10-7 mol/L.
We have not successfully constructed the recombinant plasmid with enterobactin because the gene cluster is too long.But we purified the enterobactin from E. coli successfully and tested the rust removal effect.
We intesrt the gene of DispersinB (DSPB) to pET28a and E.coli expressed DSPB successfully.
We performed enzyme activity assay and biofilm removal experiment by using cell supernatant. The enzyme activity of the supernatant is 66.363 U/mL. The supernatant has high biofilm removal rate, and the biofilm removal effect increased with time.
The fur-box is inserted into three different positions of the promoter ,named Pfur,Pfur2 and Pfur.
Recombinant E. coli successfully expressed Lysin and can can make holes in the cell membrane.
Recombinant E. coli successfully expressed cecropin AD and we test the the sterilization effect of cecropin AD .
We test the growth curve of Recombinant E. coli and found that cell growth can be inhibited after light illumination.
IRON BACTERIA-KILL CONPREHENSIVE CHARACTERIZATION
Iron bacteria is cultured in Winogradsky culture medium with 1% inoculum size from seed medium.Then medium solution is transferred into 96-well microtiter plates with different culture conditions. Then bacteria is cultured for 24 hours and OD600 is measured.
Effects of killing iron bacteria with Cecropin AD, Dsp B and enterobactin are considered comprehensively.
Dsp B is crude enzyme solution from broken cell supernatant.
Enterobacin is purified cell culture solution supernatant as what mentioned before. Cecropin AD is synthesized from Genscript. Pepide is dissolved in 1% acetic acid.