Since the current solution to the blockage is not environmentally friendly and economical, we hope to use synthetic biology to solve the three problems of rust deposition, biofilm accumulation and microbial activity by engineering microbe.
However, the three functions of engineered bacteria may bring great metabolic pressure to the engineered bacteria, and the anti-bacterial substances produced by them may adversely affect their growth and reproduction. So we need to design a set of genetic circuit to improve this situation and achieve a sequential expression of these three functions!
Inspired by the way people currently deal with it, people first add rust remover and biofilm remover, which first destroy the living environment of the microorganisms, causing them to fail to receive protection, and then bactericide is added to achieve high bactericidal effect. Therefore, in our gene circuit, it is necessary to perform the function of destroying the microenvironment of the microbial before performing the sterilization function.
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