Team:ETH Zurich/Design
Content
Introduction
Table
Model
Human Practices
Achievements
Design.
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Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
This page is different to the "Applied Design Award" page. Please see the Applied Design page for more information on how to compete for that award.
What should this page contain?
- Explanation of the engineering principles your team used in your design
- Discussion of the design iterations your team went through
- Experimental plan to test your designs
Table
Table Subtitle
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Measurement
| LUDOX CL-X | H2O | |
|---|---|---|
| Replicate 1 | 0.063 | 0.034 |
| Replicate 2 | 0.055 | 0.035 |
| Replicate 3 | 0.060 | 0.034 |
| Replicate 4 | 0.056 | 0.035 |
| Arith. Mean | 0.059 | 0.035 |
| Corrected Abs600 | 0.024 | |
| Reference OD600 | 0.063 | |
| OD600/Abs600 | 2.630 |
Particle Standard Curve Log Scale
Particle Standard Curve Linear Scale
Measurement
Sectionh1
Protocoles
Competent Cell Production (from stock)
Introduction
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware with detergent, and simply rinse it
out. Autoclaving glassware filled 3/4 with DI water is an effective way to remove most detergent residue. Media and buffers should be prepared in detergent free glassware and cultures grown up in detergent free glassware.
Materials
- CCMB80 buffer
- SOB
- Detergent-free, sterile glassware and plasticware
- Table-top OD600nm spectrophotometer
Procedure
- Prechill 250mL centrifuge tubes and screw cap tubes before use.
Evaluation
Bla Bla
Results
Did not work at all!
Competent Cell Production (from stock)
Introduction
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware with detergent, and simply rinse it
out. Autoclaving glassware filled 3/4 with DI water is an effective way to remove most detergent residue. Media and buffers should be prepared in detergent free glassware and cultures grown up in detergent free glassware.
Materials
- CCMB80 buffer
- SOB
- Detergent-free, sterile glassware and plasticware
- Table-top OD600nm spectrophotometer
Procedure
- Prechill 250mL centrifuge tubes and screw cap tubes before use.
Evaluation
Bla Bla
Results
Did not work at all!
