- We hope to repeat our plating assays to gain more conclusive results in terms of ensuring a direct correlation between the fluoride concentration and bacterial growth
- The CHOP system can be used to screen and select fluoride riboswitches with a higher affinity for fluoride, leading to more growth at lower levels of fluoride and more effective detection. This would also make the system more valuable for implementation in communities where a fluoride detection system is most needed.
- We hope to test the effectiveness of a liquid culture for our construct. Different riboswitch promoter pairs can be inserted into CHOP and response to varying levels of fluoride can be measured.
- In order to bioremediate excess levels of fluoride, we envision using fluoride riboswitches to express genes that will allow for enzymes to metabolize fluoride in the body.
- To sequester fluoride we hope to identify fluoride riboswitches with a higher affinity to fluoride and attach multiple copies of the riboswitches to nanoparticles
- For easier implementation, we hope to allow riboswitches to regulate a reporter for a more obvious sign when unhealthy levels of fluoride are present in drinking water.
- CHOP can be used to evaluate other transcriptional riboswitches that bind different metal ions, such as cobalt or nickel.