Team:Evry Paris-Saclay/Basic Part


TO CONTACT US
Genopole Campus 1, Batiment 6, 91030 Evry Cedex, France
+33 7 69 96 68 31
igemevry@gmail.com

© Copyright 2018
Design & Developpement by
IGEM EVRY GENOPOLE

BASIC PARTS

During this project, our team designed the following basic parts:


Coding sequences

Part Number Part Name
BBa_K2675000 AimR of phage phi3T
BBa_K2675001 AimP of phage phi3T
BBa_K2675002 OmpA-SAIRGA
BBa_K2675003 PelB-SAIRGA
BBa_K2675004 TAT-SAIRGA
BBa_K2675005 sfGFP
BBa_K2675006 sfGFP-LVAtag


Ribosomal binding sites (RBS)

Part Number Part Name
BBa_K2675010 Synthetic RBS designed for AimR of Phi3T
BBa_K2675011 Synthetic RBS designed for AimP of Phi3T
BBa_K2675012 Synthetic RBS designed for OmpA-SAIRGA
BBa_K2675013 Synthetic RBS designed for PelB-SAIRGA
BBa_K2675014 Synthetic RBS designed for TAT-SAIRGA
BBa_K2675015 Synthetic RBS designed for sfGFP
BBa_K2675016 Synthetic RBS designed for sfGFP
BBa_K2675017 Synthetic RBS designed for sfGFP


Promoters

Part Number Part Name
BBa_K2675020 pAimX(full) promoter of phage phi3T
BBa_K2675021 pAimX(short) promoter of phage phi3T
BBa_K2675022 pAimX(short)-J23110 hybrid promoter
BBa_K2675023 pAimX(full-1)-J23110 hybrid promoter
BBa_K2675024 pAimX(full-2)-J23110 hybrid promoter
BBa_K2675025 pAimX(full-3)-J23110 hybrid promoter
BBa_K2675026 J23110 truncated promoter (-10 only) + cloning Scar
BBa_K2675090 pAimX(full-noTerminator-v1) promoter of phage phi3T
BBa_K2675091 pAimX(full-noTerminator-v2) promoter of phage phi3T
BBa_K2675092 pAimX(full-noTerminator-v3) promoter of phage phi3T
BBa_K2675093 pAimX(full-noTerminator-v4) promoter of phage phi3T
BBa_K2675094 pAimX(full-noTerminator-v5) promoter of phage phi3T
BBa_K2675095 pAimX(full-noTerminator-v6) promoter of phage phi3T
BBa_K2675096 pAimX(full-noTerminator-v7) promoter of phage phi3T
BBa_K2675097 pAimX(full-noTerminator-v8) promoter of phage phi3T


Terminators

Part Number Part Name
BBa_K2675030 L3S2P56 Terminator
BBa_K2675031 L3S2P21 Terminator


and a Golden Gate adapter

Part Number Part Name
BBa_K2675070 Golden Gate adapter