iGEM Gifu Abstract
In vivo circular RNA expression can be a cutting edge method to perform mass-production of protein. During the translation of coding information of DNA into amino acids, function of ribosomes is naturally influential. The translation is initiated by binding ribosomes to mRNA and termination of translation is induced by a stop codon. When a start codon is recognized by ribosomes, the protein producing organelles release the protein. To produce large amount of protein and long-chain protein we can utilize circular RNA without the stop codon. iGEM Gifu 2015 performed the method of the Permuted Intron-Exon Method (PIE method). Currently 2.5% of transcribed RNA can be formed as circular RNA. With PIE method, mass-production of protein was confirmed in E.coli, however the protein had no function because of aggregation. This year our team will try to produce functional protein from the circular RNA in PURE system, a kind of cell-free system.
Welcome to iGEM 2018!
Your team has been approved and you are ready to start the iGEM season!
Before you start
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Uploading pictures and files
You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
- Have lots of fun!