Team:KAIT JAPAN

iGEM KAIT_JAPAN Abstract

We defined low metabolizable cells to be the same as interrupted animation cells and made it a goal. To control metabolism, we have focused on hydrogen sulphide (H2S) is involved in the reduction of oxygen consumption. Hydrogen sulfide have high affinity with cytochrome C oxidase which involved with oxidative phosphorylation. Inhibiting oxidative phosphorylation reversibly induces states of hypometabolism in several model organisms. Therefore, we decided to produce cystathionine γ-lyase(CTH), a hydrogen sulfide synthase, in E. coli and deliver them to eukaryotic cells. CTH has a wide temperament specificity and acts on L-cysteine to produce hydrogen sulfide when the Km value is 0.5 mM. If the concentration of hydrogen sulfide is too high, eukaryotic cells dies. Therefore, it is necessary to consider a mechanism to maintain the expression level of L-cysteine and the concentration of hydrogen sulfide constant in eukaryotic cells.

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