3' Suspects Investigating Untranslated regions on 3' endSee Our Work
what are we doing?
We are developing a new technology platform based on 3'UTR sequence characterisation to control soluble protein expression in E.coli.
We will use E.coli K12 to test our "Proof of Concept" Principle by engineering 3'UTR sequences (Sharma et al, 2011) to interact with either cytosolic proteins or 5'UTR sequences of our gene of interest: trxA and gor. Down regulation of these genes will be quantified by assessing quantitatively GFP signal. Efficiency of down regulation of genes will be quantified by assessing reaction kinetics of Alkaline Phosphatase (AP).