Welcome to the King’s College London 2018 Team Wiki!
In our project we are developing a new technology platform based on the knowledge of the role of sRNAs in bacteria to control antibiotic resistance. We will use E.coli K12 to test our strategies by engineering libraries of sRNA scaffolds to modulate gene expression patents in relation to the external environmental factors.
What are we Doing?
We are developing a new technology platform based on 3'UTR sequence characterisation to control soluble protein expression in E.coli. We will use E.coli K12 to test our "Proof of Concept" Principle by engineering 3'UTR sequences (Sharma et al, 2011) to interact with either cytosolic proteins or 5'UTR sequences of our gene of interest: trxA and gor. Down regulation of these genes will be quantified by assessing quantitatively GFP signal. Efficiency of down regulation of genes will be quantified by assessing reaction kinetics of Alkaline Phosphatase (AP).
See Our Work
Education and Outreach
As a team, we strive to reach out to all communities to share knowledge which not only empowers people of all ages, but also shares what we have learned as an iGEM team doing research in synthetic biology.
We hold many events such as school visits and iGEM symposiums, you can read more in the Human Practices section!
We document and record everything we do in the lab, so anyone can look into the research which we have been working on. In the Lab Work section you can read all about our process, testing, and model.