Welcome to iGEM 2018!
Your team has been approved and you are ready to start the iGEM season!Summary: As a new type of drug carrier, exosomes have the advantages of good biocompatibility and good bioplasticity. Currently, they have been widely used as carriers for drug development of cancer/neurological diseases, but due to low production of extracellular bodies. The difficulty of extraction has not yet been applied clinically. This project aims to use synthetic biology to induce GES-1 cells (gastric immortalized epithelial cells) to secrete exosomes, and to inhibit gastric cancer miRNA-135, miRNA-769, miRNA-942 is inserted into exosomes to rapidly produce exosomes that target gastric cancer cells and inhibit the progression of gastric cancer. First, we intend to validate miRNA-135, miRNA-769 using CCK-8, flow cytometry, and scratch assays. The tumor suppressor function of miRNA-942 inhibits cancer, and secondly constructs the lentiviral expression vector of CMV-SDC4-T2A-STEAP3-P2A-NadB-SV40 to construct a stable cell line with SDC4-STEAP3-NadB overexpression, followed by ultracentrifugation Method, WB, NTA, electron microscopy to identify exosomes, and compare the secretion difference of exosomes before and after expression, using DIO dye to label exosomes to verify the uptake ability of cancer cells, using electrotransformation to transfer miRNAs Secretion In The constructed exosomes co-cultured cancer cells, using flow cytometry, CCK-8, scratch test Developing of exosomes inhibition of cancer cells. This study intends to address the issue of exosome production, provide new ideas for the clinical transformation of exosomes, and provide a feasible solution and a preliminary basis for the clinical treatment of gastric cancer.
Before you start
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Uploading pictures and files
You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
- Have lots of fun!