Team:Lethbridge HS/Experiments

EXPERIMENTS

PROTOCOLS

Agarose Gel Extraction	Colony PCR
LB Media	DNA Purification from Enzymatic Reactions
Ligation Reactions	Ligation to Insert DNA to Vector DNA
PCR Amplification	PCR Product Purification
Phenol/Chloroform Extraction and Ethanol Precipitation	Phusion PCR Protocol
pJET Cloning	Plasmid Miniprep
Preparation of Chemically Competent Cells	Protein Overexpression
Restriction Enzyme Digest and Dephosphorylation	Transformation
Batch purification of his-tagged E.coli proteins with Ni-Sepharose	Copper binding Assay for CutA
Phage Infection of E. coli	SDS PAGE of proteins
Purification of his-tagged TruB	Expression of proteins in E. coli cells