Notebook
Week of June 18-24 2018
- Miniprep + digest to confirm GCIR1 ligation
Week of August 6-12 2018
- Double digest on DNA + gel electrophoresis
Week of August 13-19 2018
- Verify synnotch ligation via gel electrophoresis
- Maxiprep of the synnotch plasmid from successful colonies
- Tissue Culture: thawed and passaged HEK293 cells + NMuMG cells
Week of August 27-September 2 2018
- Cell lysis of transformed HEK293 cells
- Bradford assay on cell sample lysates
- Western blot
Week of September 17-23 2018
- Exposed NMuMG synnotch western blot
- Seeded HEK293 cells for transfection + froze down parental cells not used in flow cytometry
- Transfected HEK293 cells + NMuMG cells
- Performed fluorescence activated cell sorting (FACS) on transfected cells
