Welcome to Michigan State iGEM 2018!
Current Project Description
Effect of ACC deaminase engineered endophytes on plant growth in drought and high salinity conditions
Due to impending climate change related variation in rainfall, increased drought and soil salinities are predicted to become serious problems that lead to reduced crop yields from osmotic stress. In environments with high salinity and dry soil, plants respond by producing more ethylene, which engages regulatory pathways leading to premature senescence. Beneficial microbial endophytes are thought to increase the stress tolerance of plants in part by modulating signals from the ethylene pathway. For example, endophytes inside the roots of plants are known to secrete the enzyme ACC deaminase (ACCD), which converts 1-aminocyclopropane-1-carboxylate (ACC), the precursor to ethylene, into α-ketobutyrate and ammonia. The degradation of ACC reduces the formation of ethylene, thereby delaying the onset of senescence. We seek to import efficient ACCD genes into endophytic bacteria that lack this pathway. Optimized ACCD genes from bacteria and fungi will be inserted into endophytic bacteria taken from grass roots. Brachypodium distachyon, a model grass species, will be inoculated with these bacteria to compare growth to a control plant. Another focus is to identify as many transformable endophytes as possible. This will be done by collecting root samples of common grasses, harvesting bacteria, sequencing for identification, and screening for ACCD activity. ACCD genes from numerous sources will be either amplified from culture or synthesized, then assembled and inserted into endophytic grassroots bacteria. Then these bacteria will be tested on B. distachyon to show increased growth. If such engineered endophytes confer additional stress tolerance onto B. distachyon, this technique may be a useful approach to boost crop yields, especially in developing countries and other areas where irrigation practices are difficult.
Before you start
Please read the following pages:
Uploading pictures and files
You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page