Our part collection includes TonB-dependent receptor DNA sequence, 2-haloacid dehalogenase DNA sequence, Laccase DNA sequence, and also the intermediate part of 2-haloacid dehalogenase + Laccase and the final combination part of three genes. In order to let the gene can be expressed in endophyte, we bought a shuttle vector from addgene and replace the ori and also the resistance gene, which can also be seen in the basic part.
- BBa_K2546001: This part is coding for 2-haloacid dehalogenase, which can cleavage carbon-halogen bonds of halogenated compounds
- BBa_K2546004: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
- BBa_K2546007: This part is a shuttle vector with red fluorescence protein (RFP). It is composed by the Ori of pMP4657 and BBa_J364007 in pSB1C3.
- BBa_K2546008: This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the Ori of pBBR1 and BBa_J364007 in pSB1C3.
- BBa_K2546010: This part is coding for TonB-dependent receptor, which can actively transport the ferric ion carrier, siderophore, which is similar in structure with dioxin.
- BBa_K2546011: This part is coding for Laccase, which is related to lignin degradation.
- BBa_K2546002: This part is the combination of BBa_K2546001 and BBa_K2546011 which contain ribosome binding site.
- BBa_K2546005: This part is the promoter circuit of BBa_K2546002.
- BBa_K2546006: This part is the combination of BBa_K2546012 and BBa_K2546002 ,which is our ultimate goal to let three gene be expressed in one circuit.
- BBa_K2546012: This part is the promoter circuit of BBa_K2546010.
|BBa_K2546001||Coding||2-haloacid dehalogenase(HAD)||Tsu-Wang Sun||780|
|BBa_K2546002||Coding||2-haloacid dehalogenase plus laccase with robosome binding site||Tsu-Wang Sun||2408|
|BBa_K2546004||Coding||pME60C shuttle vector||Tsu-Wang Sun||6489|
|BBa_K2546005||Coding||2-haloacid dehalogenase plus laccase with promoter and ribosome binding site||Tsu-Wang Sun||2451|
|BBa_K2546006||Coding||TonB + 2-haloacid dehalogenase + laccase||Tsu-Wang Sun||4721|
|BBa_K2546007||Plasmid_Backbone||cm 4657||Tsu-Wang Sun||770|
|BBa_K2546008||Plasmid_Backbone||pME60C2 shuttle vector||Tsu-Wang Sun||5491|
|BBa_K2546009||Plasmid_Backbone||kan bbrf2||Tsu-Wang Sun||4218|
|BBa_K2546010||Coding||TonB-dependent receptor (TonB)||Tsu-Wang Sun||2244|
|BBa_K2546011||Coding||Laccase (TLcc)||Tsu-Wang Sun||1584|
|BBa_K2546012||Coding||TonB with promoter and ribosome binding site||Yu-Hsi Lin||2305|