Engineered probiotics alleviate the inflammatory bowel disease and prevent colorectal cancer
Due to the popularity of fast food and accelerated urbanization nowadays, the number of patients with inflammatory bowel disease(IBD) is rising in Asia. In addition, patients with IBD have an increased risk of developing colorectal cancer(CRC). Therefore, NEU China A aim to design a biological system against IBD and potential CRC by using engineered probiotics this year. To put out the fire in the gut, E. coli Nissle 1917 is selected as our firemen. We empower it with an anti-inflammatory device, which includes a sensor to detect the inflammatory signal, an amplifier to enhance the work ability through positive-feedback loop and an effector to secret anti-inflammatory part. Besides, we should watch out the bad guys such as cancer cells or invasive bacterial. So, we choose Saccharomyces boulardii to be our policemen. It is armed with a constitutively expressed myrosinase to turn the glucosinolates, a natural component of cruciferous vegetables, to sulphoraphane which is an organic molecule with known anticancer activity. We also equip our E. coli with this module since the sulphoraphane can inhibit the oxidative stress. In other hand, to restrict the bacterial invasion, the yeast is made to produce Lypd8, a protein represses the move of bacterial by binding to the flagella. Moreover, we decide to use a cold shock kill switch to avoid the escape of our chassis to the environment and more supporting modules will be added if necessary. We hope these modules will incorporate into a functional system eventually. Integrating probiotic therapy with synthetic biology, we envision that the collaboration between the firemen and the policemen will greatly help us to overcome the tough situation in the patient’s gut.
Welcome to iGEM 2018!
Your team has been approved and you are ready to start the iGEM season!
Before you start
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Uploading pictures and files
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When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
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- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
- Have lots of fun!