Most simplified Saccharomyces cerevisiae mitochondrial genome
It has long been a dream for synthetic biologists to artificially design and synthesize new life forms from very scratch. This year, we focused on artificial design and de novo synthesis of the most simplified Saccharomyces cerevisiae mitochondrial genome. To begin with, we designed the theoretically most simplified S. cerevisiae mitochondrial genomic sequence by means of homologous evolutionary analysis, conservative analysis and other bioinformatics tools. Then we applied the existing gene assembly technology to realize artificial de novo synthesis of the genome from the very beginning. Lack of abundancy in whole GC content and local GC clusters boils down to the complexity of the mitochondrial genome sequence, which reminds us to utilize sequence analysis software to perform targeted segmentation and splicing primer design on the genome and continuously optimize the parameters in subsequent splicing links. Cumbersome work as it is, we still have to go for it. After the combination of the genes, we planned to transform them into mitochondria-depleted Saccharomyces cerevisiae cells. And by identifying the nuances between their functions and natural mitochondria, feedback shall be received and we thus continue to optimize the design of the minimal Saccharomyces cerevisiae mitochondrial genome, completing a "Design-Synthesis-Test-Optimization" cycle.
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