Team:NPU-China/Parts

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Part overview

This year, NPU-China is committed to submitting a part library for the construction of the minimal Saccharomyces cerevisiae mitochondrial genome to the registry. We intensively characterized the part Plasmid Tightly Regulated Copy-Control System (PTRCCS) BBa_K2707007 , which we utilized to splice and stabilize our target genome. This composite part has played an egregiously critical role in our achieving the expansion of the S. cerevisiae mitochondrial genome in E. coli.

We have improved the Team NWPU’s part BBa_K2155001 this year, which encodes a BFD protein that catalyzes the conversion of formaldehyde to DHA and hydroxy acetaldehyde. We obtained the mutant F464W BBa_K2707013 of this protein via point mutation, whose function of converting formaldehyde into DHA and hydroxy acetaldehyde has been remarkably enhanced compared to the wild type.

We also submitted eight proteins that were successfully expressed by the mitochondrial genome of S. cerevisiae. Respectively, they are the three subunits of ATP synthase (atp6, atp8, atp9), cytochrome b (cob) and three subunits of cytochrome oxidase (cox1, cox2, cox3) as well as ribosomal protein (var1), among which cob and cox1 were removed all introns. Due to a lack of time and technical limitations, we did not obtain the data of these parts expressing in the mitochondria. But we have indeed gone all out to characterize these parts.

Part Table
Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry.

<groupparts>iGEM18 NPU-China</groupparts>