Team:NUS Singapore-Sci

NUS_Singapore_Science

Your team has been approved and you are ready to start the iGEM season!

Before you start

Please read the following pages:

Abstract

Recent advances in nucleic acid technology has allowed us to develop molecular tools to target and manipulate an organism's genome. Some of these advances include the ability to silence gene expression, activate gene expression and carry out epigenetic modification using CRISPR-Cas9 editing system. Major concerns for using CRISPR-Cas9 system are off-target effects and changes made in the genome could be permanent. Recent work has showed that another member of the Cas protein, Cas13 can perform similar editing functions on RNA strand. This opens up the possibility of using Cas13 to manipulate post-transcriptional activity of genes, without modification to the genome. Recently, Cox. et al (2017) successfully constructed an RNA editor in which Cas13 is fused with a deaminase enzyme ADAR, to perform A-to-I RNA editing. This finding points towards the possibility of manipulating gene expression by changing individual RNA nucleotides. However, other RNA editing systems have yet been established, our project, therefore, aims to extend the application of CRISPR-guide RNA editing on pyrimidine editing. Specifically, we will construct and characterise a synthetic biology device for targeted C-to-U RNA editing by fusing the catalytic domain of APOBEC1 enzyme and dCas13b. The development of this fusion protein holds much promise for restoring miscoded protein sequences or eliminating redundant sequences. The addition of the fusion protein could also diversify the current repertoire of RNA editing methods, potentially allowing for safer and transient treatments of genetic diseases as compared to the CRISPR-Cas9 system.

Uploading pictures and files

You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.

When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)

Wiki template information

We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!

Editing your wiki

On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!

Use WikiTools - Edit in the black menu bar to edit this page

Tips

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

  • State your accomplishments! Tell people what you have achieved from the start.
  • Be clear about what you are doing and how you plan to do this.
  • You have a global audience! Consider the different backgrounds that your users come from.
  • Make sure information is easy to find; nothing should be more than 3 clicks away.
  • Avoid using very small fonts and low contrast colors; information should be easy to read.
  • Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
  • Have lots of fun!

Inspiration

You can also view other team wikis for inspiration! Here are some examples: