Team:NUS Singapore-Sci/Parts

• Project
  • Project Overview
  • Reporter System
    • Reporter Overview
    • Dual Reporter - Demonstration
    • Dual Reporter - Improvement
    • GLB1 Reporter
  • Editor System
    • Editor Overview
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    • Kalman Filter Ensemble
    • Molecular Dynamics
    • Enzyme Kinetics
    • gRNA Design
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• Human Practice
  • Overview of Human Practice
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  • #CasAsks
    • #CasAsks: Survey
    • #CasAsks: Interview
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  • Parts

Parts

Basic Parts

Table 1. Basic BioBrick Parts

Part Name	Component	Description
BBa_K2807000	rAPOBEC1	Rat APOBEC1. Used to perform the C-to-U conversion.
BBa_K2807001	dPspCas13b	"Dead" Cas13 from Prevotella sp.. Used to target specific mRNA strands.
BBa_K2807002	PspCas13b	Cas13 from Prevotella sp.. Used to target and cut specific mRNA strands.
BBa_K2807005	EGFP	Constitutively expressed GFP reporter. Used in the Dual Colour Reporter system.
BBa_K2807006	mCherry	Red flurophore protein. Used in the Dual Colour Reporter system.
BBa_K2807007	T2A	Self cleavage peptide. Used in the Dual Colour Reporter system.
BBa_K2807008	GLB1	Human lysosomal acid beta galactosidase. Used in the GLB1 Reporter system.
BBa_K2807009	EGFP mutant reporter	EGFP with a mutated start codon (ATG -> ACG).
BBa_K2807010	GLB1 CCG mutant	Non-functional beta galactosidase with mutation of I389 to P389.
BBa_K2807011	GLB1 TCT mutant	Non-functional beta galactosidase with mutation of F107 to L107.
BBa_K2807017	EGFP with Kozak sequence	Constitutively expressed GFP reporter. Used in the GLB1 Reporter system.
BBa_K2807018	XTEN	Short linker sequence. Used in the Editor system.

Composite Parts

Table 2. Composite BioBrick Parts

Part Name	Component	Description
BBa_K2807003	rAPOBEC1-XTEN-dPspCas13b	The completed Editor system performing C-to-U conversion on targeted mRNA sequences.
BBa_K2807012	EGFP-T2A-mCherry	The completed Dual Colour Reporter system. Used to evaluate base-editing efficiency.
BBa_K2807013	EGFP-T2A-mCherry mutant	The completed Dual Colour Reporter system with single base mutation in start codon of EGFP. Used to evaluate base-editing efficiency.
BBa_K2807014	EGFP-GLB1 WT	The completed GLB1 Reporter system. Used to evaluate base-editing efficiency.
BBa_K2807015	EGFP-GLB1 CCG mutant	The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.
BBa_K2807016	EGFP-GLB1 TCT mutant	The completed GLB1 Reporter system with single base mutation which made GLB non-functional. Used to evaluate base-editing efficiency.