Organism being used: Synechococcus elongatus PCC 7942
Crude Oil and Hydrogen Sulfide
Natural crude oil is one of the primary fuel materials which has numerous uses and benefits in people’s life. As there are distinct types of crude oil produced around the world, there are differences in the quality of natural oil resources. One primary characteristic is the percentage of sulfur content in the crude oil, determining oil to be sweet or sour. Sour oil is referred to oil that contains substantial amounts of acidic gases such as hydrogen sulfide, sulfur dioxide, and carbon dioxide. Therefore, oil is preferred to be sweet with sulfur content less than 0.5%. Sulfidic contaminations in oil are problematic for a variety of reasons. Besides the fact that hydrogen sulfide is a life-threatening, toxic and flammable gas, it also damages the drilling equipment, causes corrosion of transporting pipelines and requires costly refinement methods. The issue is applicable not only to Kazakhstan, which possesses enormous oil reserves and takes 12th place in global oil production, but also to other world’s major oil producers like Canada, Russia, Mexico, Iraq and Saudi Arabia. Moreover, a release of this hazardous substance into an environment, particularly into the air, can cause respiratory and gastrointestinal irritations, poisoning, nausea, disorientation, headaches and in extreme situations can affect breathing center in the brain, causing neurological failures or death.
Treatment of crude oil
The extraction and treatment of crude oil and other fossil fuels can result in many toxic materials. One of these is Hydrogen Sulfide, generally resulting from the treatment of Sour Oil. Sour Oil is crude oil that contains a percentage of Sulfur higher than 0.5%. Hydrogen Sulfide is a dangerous gas and requires further treatment. Traditionally, Hydrogen Sulfide is eventually converted to elemental Sulfur using Chemical methods. In our project, we are developing genetically-engineered Cyanobacteria capable of coupling Sulfide oxidation, which results in elemental Sulfur and production of Hydrogen gas. The elemental Sulfur is stored inside the Cyanobacterial Biomass is then converted to Functional Materials using a Hydrothermal Process. These Functional Materials are used in applications of Solar Cells and Hydrogen Fuel Cells. We are developing a zero-waste environmentally-friendly process using Photosynthetic organisms to eliminate Hydrogen Sulfide from Fossil Fuel industrial effluent and converting the resulting Biomass into Functional Materials. The only byproduct we have is Hydrogen gas that can be used for Green Energy Applications!
The safety system, that ensures control over transformed cyanobacteria, is chromophore-assisted light inactivation SuperNova protein. The photosensitizer protein SuperNova generates Reactive Oxygen Species under regular UV light. Phototoxic sensitivity range of the protein is 500-600 nm, which will allow it to eliminate transformed species in order to protect the environment from genetically modified organisms. The protein is a monomeric form of dimeric photosensitizer protein KillerRed. Its monomeric structure ensures better localization in fusion with the target protein, which makes it more convenient to use.
Before you start
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Uploading pictures and files
You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
- Have lots of fun!