Parts Overview

Parts Used

Here we list the parts used in our study.

Name	Type	Description	Designer	Length(bp)
BBa_K2797002	Composite	Composite part for streptomycin resistance	Frank Eardley	908
BBa_K2797014	Coding sequence	Catalyses the formation of p-coumaric acid from tyrosine	Heather Bottomley	1596
BBa_K2797003	Basic	mNeonGreen fluorescent protein gene codon optimised for expression in E. coli	Kyle Stanforth	711
BBa_K2797004	Composite	Test device 1 (originally BBa_J364000) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter	Kyle Stanforth	909
BBa_K2797005	Composite	Test device 2 (originally BBa_J364001) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter	Kyle Stanforth	909
BBa_K2797006	Composite	Test device 3 (originally BBa_J364002) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter	Kyle Stanforth	909
BBa_K2797007	Composite	Test device 4 (originally BBa_J364007) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter	Kyle Stanforth	909
BBa_K2797008	Composite	Positive control (originally BBa_I20270) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter	Kyle Stanforth	910
BBa_K2797013	Plasmid vector	High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard	Kyle Stanforth	3073

Name	Type	Description	Designer	Length(bp)
BBa_I20270	Coding sequence	Positive control for the interLab study	Jason Kelly	919
BBa_J364000	Coding sequence	GFP gene for the interlab study	Traci Haddock-Angelli	918
BBa_J364001	Coding sequence	GFP gene for the interlab study	Traci Haddock-Angelli	918
BBa_J364002	Coding sequence	GFP gene for the interlab study	Traci Haddock-Angelli	918
BBa_J364007	Coding sequence	GFP gene for the interlab study	Traci Haddock-Angelli	918
BBa_J364008	Coding sequence	GFP gene for the interlab study	Traci Haddock-Angelli	918
BBa_J364009	Coding sequence	GFP gene for the interlab study	Traci Haddock-Angelli	918
BBa_R0040	Regulatory sequence	TetR repressible promoter for the interlab study	June Rhee, Connie Tao, Ty Thomson, Louis Waldman	54
BBa_J04450	Coding sequence	RFP gene for the interlab study	Tamar Odle	1069
BBa_K1033001	Coding sequence	Catalyses the reaction from p-coumaric acid to 4-coumaroyl-coenzyme A	Karl Holdar	1708
BBa_K1497000	Coding sequence	Catalyses the reaction from naringenin chalcone to naringenin.	Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen	726
BBa_K1497001	Coding sequence	Catalyses the reaction from 4-Coumaroyl-CoA to naringenin chalcone.	Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen	1197