Team:Purdue/Parts
Parts
| Part Name | Description | Type | Function | Length (bp) |
|---|---|---|---|---|
| BBa_K2696000 | Gal 1 Promoter | Regulatory | Galactose inducible promoter. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357. | 442 |
| BBa_K2696001 | Full Length Horseradish Peroxidase (HRP-FL) | Coding | A peroxidase commonly used in ELISA and related assays for its ability to catalyze reactions which convert colorless substrates like tetramethylbenzidine (TMB) to colored products. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. | 927 |
| BBa_K2696002 | Terminator 8 | Terminator | A eukaryotic terminator derived from a native terminator in Saccharomyces cerevisiae. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357. | 49 |
| BBa_K2696003 | HRP Yeast Operon | Composite | Composed of a Gal1 Promoter (Induced by galactose in absence of other energy sources), Horseradish peroxidase, and a synthetic terminator originally derived from S. cerevisiae. | 1434 |
| BBa_K2696004 | Gyp 7 Terminator | Terminator | Terminator derived from the gyp 7 gene. | 112 |
| BBa_K2696005 | His 5 Promoter | Promoter | Constitutive promoter | 500 |
| BBa_K2696006 | HRP (59-308) | Coding | Codes for the later half of horseradish peroxidase from amino acids 59 through 308. Selected for its ability to reconstitute at room temperature. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. | 753 |
| BBa_K2696007 | HRP (start + 1-58) | Coding | Codes for the first half of horseradish peroxidase from amino acids 1 through 58. It also includes a start codon. Selected for its ability to reconstitute at room temperature. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. | 117 |
| BBa_K2696008 | Igk-flag-FRP-HRP(1-58) | Coding | First part of the locking mechanism. "Igk-flag" represents the tag on the protein. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. | 585 |
| BBa_K2696009 | Igk-myc-FKB12-HRP(59-308) | Coding | Second part of locking mechanism. “Igk-myc” represents the tag on the protein. Martell, Jeffrey D, et al. “A Split Horseradish Peroxidase for the Detection of Intercellular Protein–Protein Interactions and Sensitive Visualization of Synapses.” Nature Biotechnology, vol. 34, no. 7, 2016, pp. 774–780., doi:10.1038/nbt.3563. | 1209 |
| BBa_K2696010 | pqsR | Coding | pqsR (Pseudomonas quinolone signal Receptor) is a protein capable of binding to farnesol. | 999 |
| BBa_K2696011 | Terminator 6 | Terminator | A eukaryotic terminator derived from a native terminator in Saccharomyces cerevisiae. Curran, Kathleen A., et al. “Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast.” ACS Synthetic Biology, vol. 4, no. 7, 2015, pp. 824–832., doi:10.1021/sb5003357. | 32 |
| BBa_K2696012 | TetO-Cyc1 promoter (Tet-Off) | Regulatory | When tetracycline is present, it inhibits the transcription of the gene regulated by this promoter. | 160 |
| BBa_K2696013 | TetR-VP16 | Signaling | TetR receptor fused with the C-domain of VP16 (viral protein 16), which is a transcriptional activation domain from HSV (herpes simplex virus). | 1008 |
| BBa_K2696014 | Tyrosinase | Coding | Tyrosinase is a protein capable of binding tyrosol. | 1836 |
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