With the ever-growing demand for designing proteins and enzymes with better sensitivity, selectivity, stability and affinity, oligo-based site-directed mutagenesis has become instrumental and indispensable in genetic engineering. The conventional method is considered cumbersome, for it relies on replica-plating to screen the mutants based on the reversal in resistance and sensitivity to two antibiotics: Tetracycline and Ampicillin respectively. It also necessitates sub-cloning the mutated gene in an expression vector to ultimately express the mutant-protein. Our orthogonal system facilitates fluorescence-based screening of mutants, using a novel ‘Red-Green’ Dual-Fluorescent GFP-mutant. While point-mutating the gene-of-interest, introducing a single point-mutation in the coding sequence of this GFP-mutant codes for its ‘Green-Only’ isoform. The loss of red fluorescence in the transformed colonies shall indicate successful mutagenesis. Apart from simplifying the screening method, this system facilitates the mutagenesis of the target-gene and expression of the mutated-gene using a single plasmid, thus eliminating the need for sub-cloning.

Before you start

Please read the following pages:

Styling your wiki

You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.

While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.

Uploading pictures and files

You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.

When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)

Wiki template information

We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!

Editing your wiki

On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!

Use WikiTools - Edit in the black menu bar to edit this page


This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

  • State your accomplishments! Tell people what you have achieved from the start.
  • Be clear about what you are doing and how you plan to do this.
  • You have a global audience! Consider the different backgrounds that your users come from.
  • Make sure information is easy to find; nothing should be more than 3 clicks away.
  • Avoid using very small fonts and low contrast colors; information should be easy to read.
  • Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
  • Have lots of fun!


You can also view other team wikis for inspiration! Here are some examples: