Pests have caused billions of dollars in agricultural losses. Phyllotreta striolata is one of the most serious pests of vegetable crops worldwide. At present, the major approach to control P. striolata is to use chemical pesticides, which have resulted in dietary pesticide pollution and environmental destruction, thus caused significant hazards to human health and the environment. The alternative commercial biotechnological approach to controlling insect pests relies mainly on the expression of Bacillus thuringiensis (Bt) insecticidal proteins, but Phyllotreta striolata is resistant to biopesticide Bacillus thuringiensis (Bt). Therefore, there is a critical need to develop economically, and ecologically, methods to control this this highly destructive agricultural pest. In recent years, RNAi has been used to downregulate the expression of speciﬁc genes, RNAi based transgenic plants producing dsRNAs directed against insect genes in Lepidoptera and Coleoptera pest species have been reported. In our project, we use topically application of siRNA/shRNA to control P. striolata
Zhao, Y.Y., Yang, G., Pruski, W. and You, M.S. (2008) Phyllotreta striolata (Coleoptera: Chrysomelidae): arginine kinase cloning and RNAi-based pest control. Eur J Entomol 105: 815–822.
GORDON K.H. & WATERHOUSE P.M. 2007: RNAi for insect-proof plants. Nat. Biotech.25: 1231–1232.
BAUM J.A., BOGAERT T., CLINTON W., HECK G.R., FELDMANN P., ILAGAN O., JOHNSON S., PLAETINCK G., MUNYIKWA T., PLEAU M., VAUGHN T. & ROBERTS J. 2007: Control of coleopteran insect pests through RNA interference. Nat. Biotech. 25: 1322–1326.
MAO Y.B., CAI W.J., WANG J.W., HONG G.J., TAO X.Y., WANG L.J., HUANG Y.P. & CHEN X.Y. 2007: Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol. Nat. Biotech. 25: 1307–1313.
TURNER C.T., DAVY M.W., MACDIARMID R.M., PLUMMER K.M., BIRCH N.P. & NEWCOMB R.D. 2006: RNA interference in the light brown apple moth, Epiphyas postvittana (Walker) induced by double-stranded RNA feeding. Insect Mol. Biol. 15: 383–391.
YIYING ZHAO, GUANG YANG1, GEFU WANG-PRUSKI and MINSHENG YOU. Phyllotreta striolata (Coleoptera: Chrysomelidae): Arginine kinase cloning and RNAi-based pest control. Eur. J. Entomol. 105: 815–822, 2008
Macrae IJ, Zhou K, Li F, Repic A, Brooks AN, Cande WZ, Adams PD, Doudna JA (January 2006). "Structural basis for double-stranded RNA processing by Dicer". Science. 311 (5758): 195–8..