Team:SUIS Shanghai/InterLab

Interlab

Aim:

For the 2018 Interlab, it mainly focuses on reducing the lab-to-lab variability in fluorescence measurements by normalizing to colony-forming units (CFUs) instead of OD. Our team participated in this study for creating a standardize condition for all laboratory environments.

Methods:

Transformation:
We transformed Escherichia coli​ DH5α with the plasmids that are listed below and were incubated at 37 C for 16 hours.

Calibration:

Before the cell measurement protocol, there are three sets of calibration measurements be taken. We did an OD600reference point that enable us to obtain a conversion factor to transform absorbance(Abs6​00) ​ data from plate reader into a comparable OD6​00 measurement for the spectrophotometer. A particle standard curve, help to convert Abs6​00 measurements to an estimated number of cells. In addition, a fluorescein standard curve that is necessary when comparing fluorescence output of test devices between teams.

Cell Measurement:

We measure both Abs600and fluorescence for the two colonies, with one colony at 0 hour and another at 6 hours.

CFU Counting:

We used the given formula to count colonies for two Positive Control (BBa_I20270) cultures and two Negative Control (BBa_R0040) cultures.

Results:

IGEM   |   WANYUAN   |   SUIS

Website: wanyuan.suis.com.cn

Mail Box: suisigem@outlook.com

No.509, Pingji Rd, Minhang District, Shanghai

Copyright © 2018 Shanghai United International School. All Rights Reserved.
Back To Top
Home Page
Contact