For Improvement in Gold Medal, we have improved the original GFP part (BBa_E0040) with a TCF-TATA promoter, generating a perfect reporter system for WNT ligand in our project (BBa_K2591014). It works as an improved reporter that could report even at a weak expression levels with higher efficiency. Also, we have improved the BGH polyA part (BBa_K2217015) with two LoxP sites to make a switch by Cre (BBa_K2591013) (Find more details in our part page here). We provide those well characterized and validated improved parts to the iGEM community in the hope of a broad range of synthetic biology applications.
For Validated Part in Silver Medal, we added three parts (BBa_K2591010, BBa_K2591011, and BBa_K2591012), consisting of gRNA of genes together with scaffold, all of which were well characterized, validated, and used in our project. (Find more details in our part page here)