Team:TUDelft/Acknowledgements

Sponsor

We have to thank many people that have helped us make our project become a reality. For the past nine months we received a lot of advice from various people, including our supervisors, stakeholders, sponsors and specialists.

While all of the practical work has been performed by the team the execution would not have been possible without all these people. This page is dedicated to everyone who helped us and believed in our project.

Next to everyone who helped we also have a special page for all our sponsors, without whom this project financially would not be possible.

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Principal Investigators


  • Prof. Stan Brouns
  • Prof. Timon Idema

Supervisors


  • Esengül Yildrim
  • Cristóbal Almendros Romero
  • Teunke van Rossum
  • Ana Rita Costa
  • Louis Reese
  • David Foschepoth
  • Franklin Luzia de Nóbrega
  • Sebastian Kieper
  • Zoë Robaey

General Support


  • BN Secretaries
  • For helping with booking rooms for our endless meetings, sending packages and of course for giving us our iGEM coffee card.

  • Jerwin de Graaf
  • From the communications department, for helping us with making our project movie and for his support in reaching out to the public.

  • Lenny Bakker
  • For coaching us during the media training of TU Delft and for the feedback on important publications.

  • Claire Hallewas
  • As our press officer of TU Delft, for the media support and sending the press release.

  • Abigail Sison
  • For being our iGEM Headquarters contact person and helping us with all our iGEM related questions and deadlines.

  • Michel van Baal
  • For giving us media training.

  • Mark van Papelendam
  • For helping us staying on track with our finances.

Lab support


  • Susanne Hage
  • For helping with the lab equipment and safety evaluations.

  • Sasha Khaiboulov
  • For helping with the lab equipment and making our lab a great place to work at.

  • James Brayer
  • For guiding us with the use of Oxford Nanopore Technologies.

  • Theo van Laar - "The Library"
  • For always being willing to help and giving advice. Ranging in subjects from PCR optimisation, protein expression, purification, analysis and functionality. Het also provided us with a lot of consumables we required for the development of our fusion protein. Lastly he also gave us training in using chromatography equipment.

  • Eli van der Sluis
  • For giving advice on protein expression, purification and analysis.

  • Niels van der Broek
  • For performing mass spectroscopy on our Tn5 and dxCas9 proteins.

  • Jan & Anke
  • For always providing us with autoclaved materials and also for bringing a lot of our packages.

  • Michel Bengtson
  • For providing us with some extra Blood DNA extraction kits when we needed them the most.

  • Anwar Hiralal
  • For helping us with the data analysis of our Nanopore sequencing data

Human Practices Support


  • Hanna Stammes - Science Education and Communication TU Delft
  • For giving feedback on the high school lessons we organised.

  • Jacco Gouw
  • For helping with editing of the general project movie.

  • Remko Schoot Uiterkamp
  • For scheduling several lessons on a high school.

  • Robin Sprinkhuizen
  • For scheduling several lessons on a high school.

  • Cindy Stientstra & Anne Bosma
  • For scheduling several lessons on a high school where we could teach about synthetic biology and bioethics.

  • Niek Savelkoul - RIVM
  • For giving feedback on the implementation of safety-by-design in our project.

  • Korienke Smit - RIVM
  • For being our helpful contact person at the RIVM and giving useful advice on the safety-by-design.

  • Cécile van der Vlugt - RIVM
  • For giving advice on the safety-by-design and pointing us towards possible unknown risks.

  • Pim Klaassen, Jaco Westra, Pieter van Boheemen and Lilian van Hove - RIVM and the Rathenau Institute
  • For advising us on our safety by design.

  • Olivier de Hon - Dutch Doping Authority
  • For providing us with information for the applied design during an interview.

  • Daan Bregman - TU Delft Sports Engineering Institute
  • For connecting us to the TU Delft Sports Engineering Institute and its’ network.

  • Dr. Paul Dimeo - Sterling University
  • For giving us advice on athlete behaviour and doping regulation, for bringing us in contact with many more experts and athletes and for hosting a seminar on gene doping and our project ADOPE.

  • Jeroen van Bergen & Nahuel Paolini - ImmuneTune
  • For giving us an insight into the current status of gene therapy development.

  • Arno Freeke, Luuk Goossen and Arend-Jan Krooneman - VR Institute TU Delft
  • For having interest in our VR plans and aiding in the realisation.

  • Patrick van der Duin - Stichting Toekomstbeeld der Tehcniek
  • For looking into possibilities for a cooperation.

  • Moniek Nijhuis - ex-Professional Swimster
  • For giving us her view on gene doping in an interview after having a close experience with a doping user.

  • Dr. Colin Moran - Sterling University
  • For giving us his expert opinion on genetics and sports and for giving us a labtour around the facilities they use for sports and nutrition at Sterling University.

  • Olivier Siegelaar - Professional Rower
  • For providing us with an insight on doping in rowing sports.

  • James Ashworth
  • For providing us with the sight of an professional marathon runner.

  • Everyone who filled in our survey and thereby gave us a good view on how (gene) doping fits in society

Drylab Support


  • Dr. Joost Beltman - Leiden University
  • For validating our human body kinetics model.

  • Hielke Walinga & Eduard Mucha
  • For helping and giving advice on the wiki.

  • Jacob Hesse
  • For helping with constructing our collaborations wiki page.

  • Misha Klein
  • For influencing our gRNA array model by helping us determine the number matching nucleotides required for the gRNA to bind.

  • iGEM Aberdeen 2014
  • For building a great basis for the barcoding tool that we could use and improved to make a barcoding tool perfectly fitted to our project.

  • Dr. Dorig H. Fürtinger
  • For providing advice in creating the EPO gene doping model.

Scientific Support


  • Dr. Marieke von Lindern - Sanquin
  • For bringing us in contact with Sanquin.

  • Aicha Ait Soussan - Sanquin
  • For teaching us on how to extract cell free DNA from blood plasma and serum.

  • Dr. Ellen van der Schoot - Sanquin
  • For bringing us in contact with specialists in the field of cell free DNA.

  • Onno Verhagen - Sanquin
  • For giving us a tour at Sanquin and introducing us on how Sanquin uses Next Generation Sequencing in their research.

  • Dr. Chirlmin Joo & Viktorija Blobyte
  • For giving us advice on the properties of Cas-proteins and Transposases and for validating our proof of concept of our fusion protein at the start of our project.

  • Prof. Hagan Bayley - Oxford University
  • For giving us advice on the enrichment of our samples and Oxford Nanopore Technologies.

  • Rebecca McKenzie
  • For giving advice for the MinION sequencing module.

  • Dr. Duncan McMillan
  • For advice in the early stages of the project and possible ways on how to dodge detection methods.

  • Prof. Dr. Hidde Haisma - Groningen University & Gene and Cell Doping Expert Group WADA
  • For providing us with scientific information on gene doping and current detection methods.

  • Dr. Mahadevan Lakshminarasimhan - Protein Biochemist at Modern Meadow
  • For giving advice on Cas9 purification and analysis.

  • Dr. Lixin Dai - Director of Cell Engineering Modern Meadow
  • For giving advice on Tn5 purification and analysis and also for connecting us with other experts working on Tn5.

  • Dr. William Reznikoff - Emeritus Professor University of Wisconsin-Madison
  • For giving advice on Tn5 functionality.

  • Dr. Kim Remans - Head of Protein Expression and Purification Core Facility at the European Molecular Biology Laboratory
  • For giving advice and protocols on Tn5 purification, analysis and functionality testing.

  • Dr. Anna Haagsma
  • For giving advice on Cas9 purification and analysis.

  • Dr. Ivics Zoltán - Head of Medical Biotechnology Paul Ehrlich Institute
  • For giving us recommendations on which linker to use, advice on both Tn5 and Cas9 functionality and recommendation of other experts to reach out to.

  • Dr. Lars M. Steinmetz - Senior Scientist and Director Life Science Alliance of the European Molecular Biology Laboratory
  • For sharing his research on Tn5 and for connecting us to Dr. Kim Remans and Dr. Bianca Hennig.

  • Dr. Bianca Hennig - Postdoc at the European Molecular Biology Laboratory
  • For giving advice and protocols for the Tn5 purification, analysis and functionality testing.

  • Dr. Alina Ham, Dr. Gerard Coyne & Dr. Angelica Vittori - Oxford Nanopore Technologies
  • For being our contact persons at Oxford Nanopore Technologies.

  • Dr. Marie-Eve Aubin-Tam
  • For giving us advice on nanoparticle generation, safety for working with nanoparticles and the experiments.

  • Dr. Robin Parisotto - Member UCI Athlete Biological Passport Panel
  • For giving us support and insight into the use of biological passports for doping detection.

EurAsian Meetup and Time in China


  • Ms. Dorothy Zhang
  • For her invaluable contributions in organising the iGEM EurAsian Meetup, for giving a talk and workshop on “How to iGEM” during the meetup and for helping us arrange the other visits during our time in the People's Republic of China.

  • The BGIC-Global iGEM Team 2018
  • For their help in organising the iGEM EurAsian Meetup and being a great team to mentor.

  • Dr. Wei Li
  • For the valuable interview on isolating cell free DNA and sequencing methods and for sending us literature on these topics.

  • Mr. Cao Jun
  • For the interesting conversation on the role of genetic information in sports.

  • Dr. Doris Yang
  • For giving a short opening talk at the start of the iGEM EurAsian meetup.

  • Dr. Yue (Chantal) Shen & Dr. Ying Gu
  • For giving an inspiring talk on genome-writing, eukaryotes and beyond during the iGEM EurAsian meetup.

  • Ms. Hong Chen (Rebekah) - iGEM Ambassador
  • For giving an inspiring talk on after iGEM during the iGEM EurAsian meetup.

  • Dr. Franklin Nobrega
  • For giving a great talk and workshop on How to iGEM during the iGEM EurAsian meetup.

  • Ir. Britte Bouchaut
  • For giving an inspiring talk on bioethics during the EurAsian meetup and for joining us during the full meetup to give feedback and valuable insights to all teams there.

  • Mr. Hui Kang
  • For giving an insightful talk on bioethics during the iGEM EurAsian meetup.

  • Mr. Virgil Rerimassie
  • For his guidance in designing the bioethics workshop.

  • SZU iGEM 2018 - Shenzhen University
  • For hosting us at their university to talk about iGEM and giving a great tour on their wonderful campus.

  • Yantian Senior High School workers and their volunteers
  • For making this meet-up possible not only by providing us with a location but also with help throughout the meetup.

Fundraising Help and Advice


  • Stefania Usai
  • For giving advice on our PCC grant application.

  • Simon Klein
  • For giving general advice on fundraising.

  • Jasper Veerman - iGEM TU Delft 2017
  • For giving general fundraising advice.

  • Aizo Kroon
  • For giving a training in acquisition.

  • Noa Leijdesdorff - iGEM Groningen 2018
  • For advice on running a crowdfunding.

  • Jasqueline Leemkuil & Romy van Es
  • For helping us set up the crowdfunding page.

  • Prof. Dr. Ir. Chris Kleijn
  • For valuable input on the application for the University Fund.

Other


  • The Parents of Janine
  • For lending us their vacation home in Friesland for our teamweekend.

  • Jeroen Jacques - iGEM TU Delft 2017
  • For taking our beautiful team pictures.

  • Staff of the SHANGDONG HOTEL
  • For making sure we had a pleasant stay and providing us with their personal WiFi.

  • Hajo Erwich and the Proteus ladies club eight rowing team
  • For letting us use their rowing film material.

  • De Efteling
  • For being the happiest place in The Netherlands to escape to with the team when the Wiki Freeze is approaching.

Crowdfunding


    We want to thank everyone who donated to our crowdfunding. Without this money we wouldn’t have been able to complete our project and this gave us the last necessary push!



  • Claudia
  • Beivid
  • Marcel
  • Ronald
  • Wim
  • Tatjana
  • Anonymous et al.