Interlaboratory Measurement Collaboration
In light of this year’s Interlab study, we wanted to further assist this initiative by conducting an Interlab study of our own by measuring the constructs we have designed. We wanted to investigate whether stabilized promoters would reduce variability in interlaboratory fluorescence measurements exhibited by engineered genetic constructs across different laboratories. (Jake et al.)
Fluctuations observed in plasmid copy number are caused by small changes in the cell’s environment as well as the cell itself. For example, differences in growth temperature and growth medium composition affect the copy number of plasmids. Such variations may be more apparent between labs (e.g. differences in lab equipment, handling), becoming a major hindrance in scientific research.
The measurement protocol was the same as the one performed for the plate reader measurements in this years interlab study. There were 4 Test Devices:
- TD1 and TD2 contained pupsp1 and pupsp2, two constitutive promoters .
- TD3 and TD4 contained TALEsp1 stabilized pupsp1 and TALEsp2 stabilized pupsp2 promoters.
Due to the fact that the teams that took part in our Interlaboratory Measurement had, also, participated in the iGEM Interlaboratory Measurement, so they provided us with the interlab negative control and calibration data.
The Test Devices of our Interlaboratory Collaboration
- iGEM Imperial
- iGEM Marburg
- iGEM Rice
- iGEM GIfu
Unfortunately due to time constraints we could not ask teams to repeat measurements that were affected from oversaturation. Still, we want to thank iGEM Marburg, iGEM Imperial, iGEM Rice and iGEM Gifu for their participation in our interlab collab.
iGEM Marburg Collaboration
We participated in the Interlaboratory Measurement organized by iGEM Marburg . We measured OD600 of Vibrio natriegens culture, grown at 37 degrees, at various time points. The growth curve was used to examine the growth of V. natriegens.
We participated in the OUC-China Team Collaboration by translating the comic book they created in our language in order to use this as an education material for our Public Engagement Efforts.
In collaboration with iGEM Athens, we organized the first Synthetic Biology workshop in Greece. The “SynBio Workshop” took place in Athens, in the 5th of July. Students from various backgrounds, such as medicine, engineering and mathematics, had the opportunity to participate in a hands-on workshop, designing creative solutions to real life problems using synthetic biology principles, with the help of iGEM Thessaloniki and iGEM Athens members. For more details visit our public engagement page.
We filled in surveys from
- iGEM Uppsala
- iGEM San Diego
- iGEM Bordeaux
- iGEM Bulgaria
- iGEM Imperial
From our side, we would like to thank all the iGEM Teams who forwarded our Synthetic Biology Survey to their instructors, advisors and PIs.