Team:UIOWA/Results

Results


Luminescence (RLU)/OD600 by timepoint

RLU/OD600

Peak Luminescence (RLU)/OD600

PEAK_RLU/OD600

Raw Luminosity Data (RLU)

RAW_RLU

Raw Luminosity Data by device in 3-HP

RAW_RLU_3-HP

Propionic Acid (RLU)/OD600 by timepoint

Prop_RLU/OD600

Propionic Acid (RLU)/OD600

Prop_RLU/OD600

Discussion/Analysis:

  • Induction occurred at the sixth hours proportionate to varying concentrations of 3-HP
  • Concentrations of 3-HP examined during induction:
  • 1.25 mM
  • 2.50 mM
  • 5.00 mM.
  • Non-specific induction method utilized propionic acid metabolites


Conclusion: the induction was not as expected due to lumonisty being present within the promoter construct rather than the whole 3-HP.


Future Directions:

  • Is there LysR protein assistance coming from our chassis?
  • What is the underlying biological cause of the peak and drop in luminosity?