Team:UMaryland/Parts
Parts
Our Contribution to the Registry
PETase-Linker-CBDcipA-Linker-HlyA tag + HlyBD secretion system
The PETase fusion protein is expressed on an inducible T7 promoter to be excreted extracellularly with an HlyA tag and an alpha-hemolysin secretion system (iGEM13_TecMonterrey BBa_K1166002). The PETase fusion protein is comprised of isPETase (), and a cellulose binding domain.
The PETase fusion protein is expressed on an inducible T7 promoter to be excreted extracellularly with an HlyA tag and an alpha-hemolysin secretion system (iGEM13_TecMonterrey BBa_K1166002). The PETase fusion protein is comprised of isPETase (), and a cellulose binding domain.
PcaU Sensor
Biosensor for protocatechuic acid (PCA), downstream degradation product of PET degradation. This sensor is optimized for BL21 DE3 E. coli and, when attached to a fluorescent reporter, can differentiate single micromolar PCA concentrations. PcaU itself is an inducible iclr transcription factor.
Biosensor for protocatechuic acid (PCA), downstream degradation product of PET degradation. This sensor is optimized for BL21 DE3 E. coli and, when attached to a fluorescent reporter, can differentiate single micromolar PCA concentrations. PcaU itself is an inducible iclr transcription factor.
Parts Table
| Registry Number | Part Name | Description | Link |
|---|---|---|---|
| BBa_K2825000 | PETase-Linker-CBDcipA-Linker-HlyA tag + HlyBD secretion system | The PETase fusion protein is expressed on an inducible T7 promoter to be excreted extracellularly with an HlyA tag and an alpha-hemolysin secretion system (iGEM13_TecMonterrey BBa_K1166002). The PETase fusion protein is comprised of isPETase, and a cellulose binding domain. | http://parts.igem.org/wiki/index.php?title=Part:BBa_K2825002 |
| BBa_K2825002 | PcaU Sensor | Biosensor for protocatechuic acid (PCA), downstream degradation product of PET degradation. This sensor is optimized for BL21 DE3 E. coli and, when attached to a fluorescent reporter, can differentiate single micromolar PCA concentrations. PcaU itself is an inducible iclr transcription factor. | http://parts.igem.org/wiki/index.php?title=Part:BBa_K2825002 |
Contact Us
umarylandigem@gmail.com
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umarylandigem@gmail.com
Biology - Psychology Building
4094 Campus Dr, College Park, MD 20742
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