Team:UPF CRG Barcelona/Composite
COMPOSITE PARTS
BBa_K2581004: Fatty acid acyl-CoA biosensor with weak RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581005: Fatty acid acyl-CoA biosensor with weak RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part as an intracellular biosensor of fatty acid uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581006: Fatty acid acyl-CoA biosensor with strong RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), a strong RBS (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581007: Inducible LuxR-pLux engineered device with weak RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled in order to reduce high basal activity.
It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a strong RBS (BBa_B0034), luxR repressor/activator (BBa_C0062), the double terminator again (BBa_B0015), pLux promoter (BBa_R0062) with a weak RBS (BBa_B0032) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581008: Inducible LuxR-pLux engineered device with strong RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake. Considering the leakage of the promoter being used a LuxR/pluxR lactone inducible system was coupled in order to reduce high basal activity.
It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a strong RBS (BBa_B0034), luxR repressor/activator (BBa_C0062) the double terminator again (BBa_B0015), pLux promoter (BBa_R0062) with a weak RBS (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581009: FadD gene with ATc inducible promoter with weak RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist of the expression of the own E. coli acyl-CoA Synthetase gene, FadD. This gene has an essential role in the fatty acid transportation inside the cell, as well as fatty acid activation. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a tetR repressible promoter (BBa_R0040), a weak RBS (BBa_B0032) and the the FadD gene (BBa_K258101) obtained of the own E. coli DH5-alpha strain genome.
BBa_K2581010: FadL gene with ATc inducible promoter with weak RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the own E. coli outer membrane transporter FadL. This gene has an essential role in the fatty acid transportation across the cell membrane via a transportation/acyl-activation mechanism. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a tetR repressible promoter (BBa_R0040), a weak RBS (BBa_B0032) and the FadL gene (BBa_K2581015) obtained of the own E. coli DH5-alpha strain genome.
BBa_K2581019: FadE gene with ATc inducible promoter with weak RBS
The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the acyl-coA dehydrogenase (FadE) gene which converts acyl-coA into enoyl-coA.
This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a tetR repressible promoter (BBa_R0040), a weak RBS (BBa_B0032) and the FadE gene obtained of the own E. coli DH5-alpha strain genome (BBa_K2581014).
