As Theodor Reik (Sigmund Freud disciple) said: “In science, like in all life fields, a slogan is imposed: safety first”. Nowadays, safety is an essential requirement when creating new devices which humans will use, and Printeria is not different.
This document describes the safety of our project so, a clever step before begining is to know what is it. Safety describes the containment principles, technologies and practices that are implemented to prevent the unintentional exposure to pathogens and toxines, or their accidental release . Furthermore, safety is how you have protected yoursef and other in order to avoid any harms which could occur.
Printeria is designed to be a secure device, because is going to be used by bioartists, schools and researchers which are our identified potential users according to MIT segmentation table and we do not want it to be a variable which create concern in those users. This approach toguether with our lab tools and posibilities, have influenced the experiments we did and the way we build Printeria. Next paragraphs describe our lab safety and how and why we implemented biosafety and biosecurity requirements in Printeria.
We have planned this project consequently with the tools we have. Our lab follows the current spanish legislation about risk 1 (Ley 31/1995, RD 664/1997) and as it is written, our lab is provided with an autoclave and an laminar flow cabinet with UV sterilization. Waste generated are minutely controlled by CGROM (waste management center of the Polytechnic University of Valencia). They provide every lab with special containers and instructions for the correct classification of waste which can be seen in the next photos:
Images 1-2. On the left, Roger explaining Marc how to deal with chemical containers, on the right, the containers which CGROM manage.
As an additional safety measure we use SYBR Safe instead of Eidium bromide. Its use is identical in all the protocols but without carcinogenic risks.
Access to the laboratory
As we are a multidisciplinary team formed by biotechnologists, engineers and artists, not everybody knows how to behaviour in a laboratory. That is why we did several seminars directed by Alejandro Vignoni and Yadira Boada to teach the basics of their laboratory. However, as a security measure, they decided that not everybody would be allowed to work in the lab alone, only biotechlogists and biomedical engineers could be there without surveillance due to their experience and knowledge adquiered during their degree.
Over the course of these seminars we were informed about the evacuation protocols, waste management and our rights and liabilities as UPV staff. These information can be seen in the information sheets they gave us and that you can download through these buttons:
Printeria uses E.coli and V.natriegens as chassis. Both are classified as risk 1 so the harm which can cause to people is minimal. The E.coli are from strains DB3.1, 10G and TOP10, for V.natriegens we use the strain ATCC 1404 from CECT (Colección Española de Cultivos Tipo). To do constructions we use biobricks and parts supplied by IDT prepared with the Golden Gate grammar.
1. Dual use: A report by iGEM team Bielefeld-CeBiTec 2015. [Germany]. Universität Bielefeld, . 30 pages.