Team:XJTLU-CHINA/Composite Part
Composite Parts
| Part Number | Part Name | Function | Length | Designer |
|---|---|---|---|---|
| BBa_K2619100 | Exosome marker | CD63 is fused with nluc to the measure the quantity of exosome by luminescence signal shown by nluc. | 1241bp | Haoping Cheng |
| BBa_K2619101 | Packaging device (L7Ae) | L7Ae is linked to the C-terminus of CD63 to package mRNA with C/D box during the formation of exosomes. | 1113bp | Haohui Fang |
| BBa_K2619102 | Packaging device (MS2) | MS2 can be fused with exosome-associated proteins CD63 to package mRNA with 3 loops during the formation of exosome. | ||
| BBa_K2619103 | Booster | The tricistronic STEAP3-SDC4-NadB construct at a 1:1:1 ratio by which exosome secretion is supposed to enhanced by 15-fold to 40-fold in yield | 2682bp | Shiyu Liu |
| BBa_K2619104 | mRNA modification (nluc-C/D box-VEEV) | C/D box-VEEV can be added to 3'-UTR region of actively loading RNA to bind to RNA binding domain of packaging device with L7Ae and stablize mRNA. Nluc was added as reporter gene. | 1016bp | Shiyu Liu |
| BBa_K2619105 | mRNA modification (nluc-3 loop-VEEV) | C/D box contains the structure of k-loop RNA motif which can be recognized by L7Ae. By engineering it at the 3'-UTR of cargo mRNA, CD63-L7Ae can load that RNA into exosome. | 72bp | Peng Chai |
| BBa_K2619006 | 3 loop | 3 loop-VEEV can be added to 3'-UTR region to actively load RNA by binding domain of packaging device with MS2.Nluc was added as reporter gene and VEEV is utilized for stablization. | 1033bp | Shiyu Liu |
| BBa_K2619108 | Modified RVG-Lamp2b | GNSTM was added to 5' of RVG to increase the drug-delivery efficiency of this targeting device. | 1429bp | Haohui Fang |
| BBa_K2619109 | nluc-C/D box | This part is used for measure the packaging ability of CD63-L7Ae--exosomal membrane fusion protein by fusing C/D box with reporter gene. | 590bp | Miaoyan Li |
| BBa_K2619014 | nSMase with Kozak | Exosome production boosting device. | 1987bp | Haohui Fang |
Collaborators and Supporters
Location
Rm 363, Science Building
Xi'an Jiaotong-Liverpool University
111 Ren'ai Road, Suzhou, China
215123
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Get in touch
igem@xjtlu.edu.cn