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<li>Inoculate a 300 ml shake flask containing 100 ml LB medium with the overnight culture to an OD<sub>6 <li>Inoculate a 100 ml shake flask containing 10 ml LB medium with the overnight culture to an OD<sub>60

...<p>Devices: three triangular flasks, a centrifugal cup (50ml), two shake tubes,</p> <p>2.1 Shake tube, triangular flask, centrifuge cup, rinse with ddH2O repeatedly without

...sform:translateX(10px)}}.shake{-webkit-animation-name:shake;animation-name:shake}@-webkit-keyframes swing{20%{-webkit-transform:rotate(15deg);transform:rota @-webkit-keyframes shake {

Inoculatedd DH5a escherichia coli in LB liquid medium and shake overnight. 2 Inoculatedd DH5a escherichia coli in LB liquid medium and shake overnight.

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<div class="zhengwen">2. Shake bacteria fluid transformed with part 10，part 12，part13，part17 on the <div class="zhengwen">2. Shake bacteria fluid transformed with piGEM2018-Module003 on the shaking table 37

@-webkit-keyframes shake { @-moz-keyframes shake {

<div class="zhengwen">2. Shake bacteria fluid transformed with part 10，part 12，part13，part17 on the <div class="zhengwen">2. Shake bacteria fluid transformed with piGEM2018-Module003 on the shaking table 37

@-webkit-keyframes shake { @-moz-keyframes shake {

...After drying, add 1 L ultrapure water, then add 2 ml DEPC in a fume hood, shake overnight at 37 °C, sterilize at 121 °C for 30 min, and then store at roo ...glacial acetic acid, and then constant volume to 100 mL. Add 200 μl DEPC, shake overnight at 37 °C, sterilize at 121 °C for 45 min, and then store at roo

<li>Shake until dissolved.</li> <li>Shake until dissolved.</li>

Shake at 30℃ to OD600nm = 0.6<br> Shake at 30℃ to OD600nm = 0.6<br>

...ll">SbsB WT2 & SBSS &Delta;N in 20ml LB fluid with 20&mu;l Amp inoculated, shake overnight at 37°C. Next day: undying with 10% acetic acid (while shaking)< • Shake membrane with secondary antibodies (1: 10000, 20ml blocking buffer + 2&mu;l

...T). For 0.75 mm-thick gels, shake for 10 to 15 min; for 1.5 mm thick gels, shake for 30 to 60 min.<br> ...off fixing solution, cover over with Coomassie blue staining solution and shake at RT for 2 h.<br>

<p>2. Add 200 μl of buffer GA to the cell pellet and shake until the cells are completely suspended.</p> ...>[2]If RNA needs to be removed, add 4 μl of RNase A (100 mg/ml) solution, shake the mixture for 15 seconds, and leave the mixture at room temperature for 5

...TOP 10②. The colonies were picked and inoculated into liquid LB mediums. Shake the bacterial fluids at 37°C overnight (12 hours).</p> <p>Bacterial fluid (Blank/PA): shake for 12 hours</p>

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section 4.3 for more information). Shake at 37 °C and ~ 300 rpm for ~ 8 h. <li>Place at 37°C for 60 minutes. Shake vigorously (250 rpm) or rotate.</li>

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