Team:Goettingen/Notebook/evolution April
Used recipes for buffers and media
25.04.18
LB-Medium
| Components | Amount (g) |
|---|---|
| Tryptone | 10 |
| NaCl | 10 |
| Yeast exctract | 5 |
| 1000 mL H2O | |
| Autoclavation |
SP-Medium
| Components | Amount (g) |
|---|---|
| Nutrient broth | 8 |
| MgS04 ● 7 H2O | 10 |
| KCl | 1 |
| 1000 mL H2O | |
| Autoclavation | |
| CaCl2 | 0.5 M |
| MnCl2 | 10 mM |
| Iron ferric ammonium citrate | 2 mL |
10× MN-Medium
| Components | Amount (g) |
|---|---|
| K2HPO4 ● 3 H2O | 136 |
| KH2PO4 | 60 |
| Na citrate | 10 |
| 1000 mL H2O | |
| Ad to 50 mL with sterile water | 100 |
27.04.18
Cryo cultures of strains 168, BP233, BP234 were prepared with 90% LB medium and 10% DMSO. The cells were frozen and stored at -80°C.