Improvement

Based on Harvard_BioDesign team in 2016

Similarities

Both our project has focus on PET decomposition. The coding sequence of PETase and MHETase from Ideonella Sakaiensis was transformed to e coli for faster expression.

Better utilisation of ethylene glycol

In the Harvard_BioDesign team’s design, the ethylene glycol is collected in a separate collection box. However, as ethylene glycol is a harmful substance [1] and can be utilised better. Hence, we propose using the ethylene glycol pathway in e coli [2]to further decompose ethylene glycol to carbon dioxide.

Different focus of plastic waste

Harvard_BioDesign team focused on plastic waste in the ocean while we wish to apply our design on an industry scale.

Based on SCU_China in 2015

Use of nif genes

SCU_China used nif genes for nitrogen fixation, while we amino acid substituted the nif genes to change the function of the genes.

Method of Gibson assembly

SCU_China wanted to do Gibson assembly in one go, but we made a large construct with separated steps.

References

[1]	National Center for Biotechnology Information, "Ethylene Glycol," [Online]. Available: https://pubchem.ncbi.nlm.nih.gov/compound/ethylene_glycol#datasheet=lcss&section=Top. [Accessed 17 October 2018].
[2]	I. M. K. A. M. M. P.-G. A. S.-Z. S. G.-C. C. B.-M. C. F. A. M. H. A. K. M. Krummenacker, "Escherichia coli K-12 substr. MG1655 Pathway: ethylene glycol degradation," [Online]. Available: https://biocyc.org/ECOLI/NEW-IMAGE?type=PATHWAY&object=PWY0-1280. [Accessed 17 October 2018].

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Team:HKJS S/Improve