Team:Mingdao/Human Practices 2-2-2

HIV Testing

Interview: Prof. Jason C. Huang
Date: Jul. 29, 2018
Location: AIDS Control Center at National Yang Ming University, Taipei
Reflection: HIV control, T cell activation signaling

Why: To solve the problem we met in our experiment concerning the Toll signaling with synthetic CD4 receptor

How: Today, we headed to Taipei to have a meeting with professor Huang, who works at the AIDS Control Center at National Yang Ming University.

What:

We presented our project. In brief, we hypothesized that AMP promoter can be induced by CD4 dimer to generate GFP signal. Then by adding the HIV antigen gp120, it will destroy the originally formed dimer and inhibit the signaling pathway. He provides his comments explaining why our project met a problem. Below are some pieces of advice from him:
1.Simply add LPS to your cells expressing Toll to check whether NF-kB signal can be delivered to AMP promoter
2.Check if the gene codon usage between human and mosquitoes is compatible.
3.Use anti-CD4 antibody to confirm that CD4-Toll chimera can form dimers and induce the signaling.
4. Run a Western SDS-PAGE to check if CD4 could form dimers in your system.

Reflection:

Take into practices. Discuss the ideas with our instructor Dr. Chen. We design a new experiment based on the comments from Prof. Huang. We planned to bought a CD4 antibody to confirm CD4 dimer and the signaling in response to HIV gp120.

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