Team:Munich/PhageAssembly5.html
T4 Phage assembly
2018/10/08- 2018/10/09| Participants: | Brigit Tunaj |
| Protocol: | Phage Assembly TXTL |
| Notes: | Phages were assembled in commercial cell extract. |
| Results: | Assembled phages were able to create plaques. |
TEM Imaging of Phages
2018/10/08- 2018/10/17| Participants: | Brigit Tunaj |
| Protocol: | TEM probe preparation |
| Notes: | Phages were concentrated from lysate supernatants and prepared for TEM Imaging. Samples imaged: P2-Phage, P3-Phage, T5-Phage, 33-Phage, T4 Phage, T7 phages-infected bacteria and T4 phage-infected bacteria |
| Results: | Beautiful pictures |
Purification of synthetic phages
2018/10/10- 2018/10/13| Participants: | Brigit Tunaj |
| Protocol: | Amicon purification of Phages, TEM probe preparation |
| Notes: | no notes |
| Results: | There were no synthetic phages visible in the TEM. Purification did not work. |
DNA Methylation of T7 phage DNA for modular Phage Genome assembly
2018/10/01- 2018/10/03| Participants: | Brigit Tunaj |
| Protocol: | DNA methylation, DpnI Digestion, Agarose gel |
| Notes: | DpnI digestion was performed to test DNA methylation |
| Results: | Worked. Agarose Gel was empty after methylation and DpnI digestion. |