Team:Munich/mProteinEn.html
Modular Protein Engineering
during Wikifreeze| Participants: | Quirin Emslander |
| Protocol: | Phage T7 DNA Purification, Phage T7 Assembly TX-TL | Notes: |
Overexpression of proteins Purification and Assembly of proteins |
Results: |
Plasmids psb1C3_T7_His_yFP_HOC and psb1C3_T7_His_Nanoluc_HOC got cloned. Proteins His_yFP_HOC / His_Nanoluc_HOC got expressed and purified. Phages assembled with His_yFP_HOC / His_Nanoluc_HOC |
Assembly of T4 Phage with Yfp_his_hoc in commercial cell extract
2018/10/10| Participants: | Brigit Tunaj |
| Protocol: | Phage Assembly TX/TL, Agar overlay plaque assay | Notes: | 600 ng DNA and 0,54 uM yfp_his_hoc Protein | Results: | Spot test worked --> has been used fluorescence microscopy |