Team:Nottingham/Composite Part

Clostridium dTox Project

Project description Abstract

Human Practices

Overview Silver Gold Safety and Ethics

Public Engagement

Discovery day School workshops Media

Lab

Ideas Design Results Conclusion Future Work Interlab Labfolder

Modelling

Introduction Summary Lytic Phage Models Temporate Phage Models Comparsion of all three models Methodology Parameters References

Collaborations

The University of Warwick Team BioMarvel- Korea The University of Warwick and Imperial College London UK Meetups

Achievements

Medal Criteria Parts Improved parts Demonstrate General conclusion

Team

Students Supervisors Principal Investigators

Attributions

This is part of the Achievements page!

To meet medal criteria, and to make life for judges easier, we have extracted this part of the achievements page.
Original page

Parts

Unique part name	Part type	Description
BBa_K2715009	Basic	C. acetobutylicum thiolase gene RBS
BBa_K2715010	Basic	Constitutive promoter from C. acetobutylicum thiolase gene
BBa_K2715011	Basic	Constitutive promoter from C. sporogenes ferrodoxin gene
BBa_K2715012	Basic	Native promoter from C. difficile toxin A gene
BBa_K2715013	Basic	Native promoter from C. difficile toxin B gene
BBa_K2715014	Basic	HydA Terminator from C. acetobutylicum
BBa_K2715015	Basic	Antisense RNA targeting tcdA 1
BBa_K2715016	Basic	Antisense RNA targeting tcdA 2
BBa_K2715017	Basic	Antisense RNA targeting tcdB 1
BBa_K2715018	Basic	Antisense RNA targeting tcdB 2
BBa_K2715019	Basic	C. acetobutylicum thiolase promoter 5' UTR
BBa_K2715020	Basic	C. sporogenes ferredoxin gene RBS
BBa_K2715021	Basic	C. difficile Toxin A promoter 5' UTR
BBa_K2715022	Basic	C. difficile Toxin A promoter RBS
BBa_K2715023	Basic	C. difficile Toxin B promoter 5' UTR without RBS
BBa_K2715024	Basic	C. difficile Toxin B promoter RBS
BBa_K2715032	Basic	Synthetic guide RNA targeting sequence 1 for tcdA promoter
BBa_K2715033	Basic	Synthetic guide RNA targeting sequence 2 for tcdA promoter
BBa_K2715034	Basic	Synthetic guide RNA targeting sequence 3 for tcdA promoter
BBa_K2715035	Basic	Synthetic guide RNA targeting sequence 4 for tcdA promoter
BBa_K2715036	Basic	Synthetic guide RNA targeting sequence 5 for tcdA promoter
BBa_K2715037	Basic	Synthetic guide RNA targeting sequence 6 for tcdA promoter
BBa_K2715042	Basic	Synthetic guide sequence for S. pyrogenes cas9 - targeting sequence
BBa_K2715043	Basic	Alternative sigma factor TcdR regulating toxin expression in C. difficile
BBa_K2715001	Composite	Constitutive clostridial thiolase promoter BBa_K2715010, strong RBS and GFP reporter
BBa_K2715002	Composite	Constitutive clostridial ferredoxin promoter BBa_K2715011, strong RBS and GFP reporter
BBa_K2715003	Composite	Clostridial promoter of tcdB with its native 5' UTR and RBS, driving a GFP reporter
BBa_K2715004	Composite	Clostridial promoter of tcdA with its native 5' UTR and RBS, driving a GFP reporter
BBa_K2715005	Composite	E.coli promoter BBa_J23119 driving synthetic guide 1 targeting tcdB promoter
BBa_K2715006	Composite	E.coli promoter BBa_J23119 driving synthetic guide 2 targeting tcdB promoter
BBa_K2715007	Composite	Antisense RNA targeting C. difficile toxins composite 1
BBa_K2715008	Composite	Antisense RNA targeting C. difficile toxins composite 2
BBa_K2715025	Composite	Constitutive clostridial promoter BBa_K2715010, strong RBS and GusA reporter
BBa_K2715026	Composite	Constitutive clostridial promoter BBa_K2715011, strong RBS and GusA reporter
BBa_K2715027	Composite	Clostridial promoter of tcdB with its native 5' UTR and RBS, driving a GusA reporter
BBa_K2715028	Composite	Clostridial promoter of tcdA with its native 5' UTR and RBS, driving a GusA reporter
BBa_K2715029	Composite	Constitutive E.coli promoter BBa_J23106, strong RBS and GusA reporter
BBa_K2715030	Composite	Constitutive E.coli promoter BBa_J23114, strong RBS and GusA reporter
BBa_K2715031	Composite	Constitutive E.coli promoter BBa_J23119, strong RBS and GusA reporter
BBa_K2715038	Composite	E.coli promoter BBa_J23119 driving synthetic guide 3 targeting tcdB promoter
BBa_K2715039	Composite	E.coli promoter BBa_J23119 driving synthetic guide 4 targeting tcdB promoter
BBa_K2715040	Composite	E.coli promoter BBa_J23119 driving synthetic guide 5 targeting tcdB promoter
BBa_K2715041	Composite	E.coli promoter BBa_J23119 driving synthetic guide 6 targeting tcdB promoter
BBa_K2715044	Composite	Constitutive expression of dCas9, and regulatory region of Toxin A in C. difficile driving GusA
BBa_K2715106	Composite	Constitutive E.coli promoter BBa_J23106, strong RBS and GFP reporter
BBa_K2715114	Composite	Constitutive E.coli promoter BBa_J23114, strong RBS and GFP reporter
BBa_K2715119	Composite	Constitutive E.coli promoter BBa_J23119, strong RBS and GFP reporter