Team:Queens Canada/liquid

Liquid Culture · Benchling

Liquid Culture

Introduction

Liquid Culture from the streaked plates.

Materials

    • 10uL of Kanamycin/tube (1uL:1mL... with the broth)
    • 10mL of LB broth/tube
    • Pipette tips

Procedure

  1. Fill conical tube with LB broth.
  1. Label the tubes: e.coli type, antibiotic, plasmid
  1. Add antibiotic
  1. Take 100uL pipette tip, gently swipe a colony
  1. Drop in tube, shut.
  1. Store overnight, 37C, in shaker.
  • NOTES
  • Store the petri dishes in 4C once used. For long term use, seal with parafilm.
  • Restreak every month to ensure the colonies live.