Team:Queens Canada/lysis

Lysis by Sonication

Project: QGEM Team

Authors: Queens IGEM

Date: 2018-07-31

Tuesday, 7/31/18

1.

Pellet 1-5mL of liquid culture at 16g for 1 min

2.

Remove Excess LB

3.

Add Phosphate Lysis Buffer (Allingham Lab Recipe)

4.

Wait 5 minutes

5.

Sonicate for 9 seconds

6.

Place on ice immediatly

7.

Ethanol and ddH2O off the microtip between trials.

8.

Sonicate again for 9 seconds

9.

Pellet at 16g for 1 min

10.

Collect and test supernatant