Team:Thessaloniki/Notebook
Notebook
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We presented our project idea to our Primary Investigators and Professors
of our Faculties. During this time period we focused our brainstorming
sessions on the enrichment of our project, we enlisted the materials needed
for the laboratory work and we began to develop our experimental workflow.
During our first weeks in the lab we received Safety and Security training by various members of the labs we worked in. We were taught proper lab practice, sterile techniques and physical biosecurity, along with proper waste disposal and emergency procedures. We were instructed on the function and usage of the various equipment in the lab like Biosafety Cabinets, centrifuges and more.
- General Laboratory Procedures
- Equipment Use
- Emergency response procedures
- Prevention and control of laboratory contaminations
- Handling of hazardous reagents
- Disposal of Buffers and Chemicals
Setting up the Lab This week we focused on gathering the material and
equipment needed for our laboratory work. We prepared solutions, agar
plates, Bacterial Growth Medium and a small batch of DH5a competent cells.
We also resuspended the parts we needed from the iGEM 2018 distribution
kit.
Wet Lab-Dry Lab meetings focused on Human Practices
This week we continued setting up the lab and gathering the materials
needed for our experiments. We, also, conducted the Interlaboratory
Measurement Study.
The pThSSe_59, pThSSe_60 plasmids from Addgene and our first gBlocks
arrived. We focused on cloning the Talesp1 and Talesp2 stabilized cassette
in backbones with different ORIs and prepared the constructs for Flow
Cytometry.
We successfully removed the two illegal PStI sited inside the pTHSSe_59 and
pTHSSe_60 plasmids.
We got our first results for the TAL Effector Stabilization system.
We focused on the design of our CRISPRi stabilization system.
This week we successfully cloned our Riboswitch Constructs and got our
first Flow Cytometry data. We, also, successfully cloned our Theophylline
Riboswitch in the Tale Stabilised cassette.
After a constructive wet-dry meeting we decided to go a step further and
design a RNA level control system. Together with the dry lab we simulated
different topologies and chose the one better suited for our system.
This Week we started working on our AND Gate Constructs.
Our Interlaboratory Measurement Collab has started. We prepared and
measured the samples to be sent to the other iGEM Teams.
The pAN-PTet-dCas9 plasmid arrived and we started working on our CRISRi
stabilization system. The AND Gate gBlocks arrived.
We received our sequencing results
We conducted the flow cytometry measurements for our constructs and
analyzed the results.
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